2 documents found, page 1 of 1
Proteomic analysis of the action of the Mycobacterium ulcerans toxin mycolacton...
Gama, José B.; Ohlmeier, S.; Martins, Teresa G.; Fraga, Alexandra G.; Marques, Belém Sampaio; Carvalho, M. Alice; Proença, M. Fernanda R. P.Buruli ulcer (BU) is a neglected tropical disease caused by Mycobacterium ulcerans. The tissue damage characteristic of BU lesions is known to be driven by the secretion of the potent lipidic exotoxin mycolactone. However, the molecular action of mycolactone on host cell biology mediating cytopathogenesis is not fully understood. Here we applied two-dimensional electrophoresis (2-DE) to identify the mechanisms ...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a specific substrate of yea...
Silva, Alexandra; Almeida, Bruno; Marques, Belém Sampaio; Reis, M. I .R.; Ohlmeier, S.; Rodrigues, Fernando José dos Santos; Vale, Ana doYeast metacaspase (Yca1p) is required for the execution of apoptosis upon a wide range of stimuli. However, the specific degradome of this yeast protease has not been unraveled so far. By combining different methodologies described as requisites for a protein to be considered a protease substrate, such as digestome analysis, cleavage of recombinant GAPDH by metacaspase and evaluation of protein levels in vivo, ...