7 documents found, page 1 of 1
Prevalence of horizontal alveolar changes in edentulous patients: a retrospecti...
Dias,Márcio Américo; Romito,Giuseppe; Villar,Cristina Cunha; Sapata,Vitor Marques; Teixeira,Marcelo Lucchesi; Aloise,Antonio Carlos; Mannina,CarolinaAbstract Horizontal bone loss after tooth extraction is a common finding that demands bone reconstruction in various cases. The aim of this study was to assess the horizontal alveolar status in partially and completely edentulous patients using cone-beam computed tomography (CBCT). In total, 1516 CBCT scans of 1404 adult patients were analyzed. Assessment of the images was performed in accordance with the previ...
Horizontal Bone Reconstruction on sites with different amounts of native bone: ...
Pelegrine,André Antonio; Romito,Giuseppe; Villar,Cristina Cunha; Macedo,Luís Guilherme Scavone de; Teixeira,Marcelo Lucchesi; Aloise,Antonio CarlosAbstract: The lack of guidelines for bone augmentation procedures might compromise decision making in implantology. The objective of this study was to perform a retrospective study to verify the outcomes of horizontal bone reconstruction in implant dentistry with different types of materials and amounts of native bone in the recipient bed to allow for a new guideline for horizontal bone reconstruction. One hund...
TGF-β1 on induced osteogenic differentiation of human dermal fibroblast
Aloise,Antonio Carlos; Pereira,Max Domingues; Duailibi,Silvio Eduardo; Gragnani,Alfredo; Ferreira,Lydia MasakoPURPOSE: To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS: We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 mmol/l of β-glycer...
Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats
Suartz,Caio Vinicius; Gaiba,Silvana; França,Jerônimo Pereira de; Aloise,Antonio Carlos; Ferreira,Lydia MasakoPURPOSE: To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.METHODS: Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5). ADSC were separated, cultured and then analyzed. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed. After the surg...
Development of experimental in vitro burn model
Fernandes,Ana Carolina Morais; França,Jerônimo Pereira de; Gaiba,Silvana; Aloise,Antonio Carlos; Oliveira,Andrea Fernandes dePURPOSE: To propose an experimental burn model in NIH-3T3 cell line.METHODS:Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS:The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 wa...
Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal s...
Suartz,Caio Vinicius; Gaiba,Silvana; França,Jerônimo Pereira de; Aloise,Antonio Carlos; Ferreira,Lydia MasakoPURPOSE: To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC). METHODS: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ...
Keratinocyte growth factor protected cultured human keratinocytes exposed to ox...
Gragnani,Alfredo; Rossi,Marina Bertelli; Albuquerque,Janne Cely Medeiros; Brito,Gabriela Soares Silva; Aloise,Antonio Carlos; Ferreira,Lydia MasakoPURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment ...