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Production and characterization of protein-rich extracts from the red macroalga...
Mateus, Marília; Mão de Ferro, Rita; Pinheiro, Helena M.; Machado, Remígio; R. da Fonseca, M. Manuela; Sapatinha, Maria; Pires, Carla; Marmelo, IsaThe red macroalga Gelidium corneum is a species commercially exploited for agar extraction, though with enormous potential for other industrial applications. This work aimed to produce protein-rich extracts from the crude alga and from residues of the industrial agar extraction process by applying different extraction and recovery procedures, and to characterize the obtained protein extracts. The sequential imp...
Ulva rigida valorization into poly(3-hydroxybutyrate), organic acids and functi...
Leandro, Tânia; Teles, Marco; Dias, Joana Sofia Gomes; Marques, Mafalda; Rocha, Cristina M. R.; da Fonseca, M. Manuela R.; Cesário, M. TeresaHalomonas elongata 1H9T is a moderate halophilic strain able to produce poly(3-hydroxybutyrate) (P(3HB)), a biodegradable plastic, and gluconic acid, a valuable organic acid with wide industrial applications. In this work, the green alga Ulva rigida was used as platform to produce cultivation substrates for microbial conversion as well as functional ingredients, targeting its full valorization. The liquor obtai...
Xylose metabolism in bacteria : opportunities and challenges towards efficient ...
Domingues, Rafael; Bondar, Maryna; Palolo, Inês; Queirós, Odília; Almeida, Catarina Dias de; Cesário, M. TeresaIn a sustainable society based on circular economy, the use of waste lignocellulosic biomass (LB) as feedstock for biorefineries is a promising solution, since LB is the world’s most abundant renewable and non-edible raw material. LB is available as a by-product from agricultural and forestry processes, and its main components are cellulose, hemicellulose, and lignin. Following suitable physical, enzymatic, and...
Marine algal carbohydrates as carbon sources for the production of biochemicals...
Cesário, M. Teresa; Fonseca, M. Manuela R. da; Marques, Mafalda M.; Almeida, M. Catarina. M. D. deThe high content of lipids in microalgae (>60% w/w in some species) and of carbohydrates in seaweed (up to 75%) have promoted intensive research towards valorisation of algal components for the production of biofuels. However, the exploitation of the carbohydrate fraction to produce a range of chemicals and chemical intermediates with established markets is still limited. These include organic acids (e.g. succi...
A Burkholderia sacchari cell factory: production of poly-3-hydroxybutyrate, xyl...
Raposo, Rodrigo S.; Almeida, M. Catarina M. D. de; Fonseca, M. Manuela da; Cesário, M. Teresa"Efficient production of poly-3-hydroxybutyrate (P(3HB)) based on glucose-xylose mixtures simulating different types of lignocellulosic hydrolysate (LCH) was addressed using Burkholderia sacchari, a wild strain capable of metabolizing both sugars and producing P(3HB). Carbon catabolite repression was avoided by maintaining glucose concentration below 10g/L. Xylose concentrations above 30g/L were inhibitory for ...
Feeding strategies for tuning poly (3-hydroxybutyrate-co-4-hydroxybutyrate) mon...
Raposo, Rodrigo S.; Almeida, M. Catarina M. D. de; Fonseca, M. M. R. da; Cesário, M. TeresaPoly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-4HB)) co-polymers were produced at bench-scale in fed-batch cultivations by Burkholderia sacchari from glucose (main carbon-source) and gamma-butyrolactone (GBL) as co-substrate. As P(3HB-4HB) properties highly depend on the 4-hydroxybutyrate (4HB) molar fraction, it is advantageous to have a thorough knowledge of the process in order to promote the production...
Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulos...
Cesário, M. Teresa; Raposo, Rodrigo S.; Almeida, M. Catarina M. D. de; Keulen, Frederik van; Ferreira, Bruno S.; Fonseca, M. Manuela R. da"Polyhydroxyalkanoates (PHAs) are bioplastics that can replace conventional petroleum derived products in various applications. One of the major barriers for their widespread introduction in the market is the higher production costs when compared with their petrochemical counterparts. In this work, a process was successfully implemented with high productivity based on wheat straw, a cheap and readily available ...
Production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sac...
Cesário, M. Teresa; Raposo, Rodrigo S.; Almeida, M. Catarina M. D. de; Van Keulen, Frederik; Ferreira, Bruno S.; Telo, João P."Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production. Using a similar feeding strategy developed for P(3HB) production based on WSH, fedbatch cultures were developed aiming ...