167 documents found, page 1 of 17
Screening and in silico characterization of prophages in Helicobacter pylori cl...
Ferreira, Rute Vanessa Novais; Pinto, Graça; Presa, Eva; Oleastro, Mónica; Silva, Catarina; Vieira, Luís; Sousa, Cláudia Sofia CunhaThe increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique test...
Source attribution of human infection: a multi-country model in the European Union
Thystrup, Cecilie; Brinch, Maja Lykke; Henri, Clementine; Mughini-Gras, Lapo; Franz, Eelco; Wieczorek, Kinga; Gutierrez, MontserratIntroduction: Infections caused by spp. represent a severe threat to public health worldwide. National action plans have included source attribution studies as a way to quantify the contribution of specific sources and understand the dynamic of transmission of foodborne pathogens like and . Such information is crucial for implementing targeted intervention. The aim of this study was to predict the sources of hu...
Probe-based metagenomic pathogen detection: advancing laboratory capacity for c...
Ferreira, Rita; Coelho, Luís; Santos, João Dourado; Sobral, Daniel; Isidro, Joana; Mixão, Verónica; Pinto, Miguel; Nunes, Alexandra; Borrego, Maria JoséProbe-based pathogen enrichment, followed by NGS, is a promising tool for complex diagnosis, overcoming traditional challenges of shotgun metagenomics, namely small microbial/human genetic material ratio and demanding computational resources. Here, we assessed the combined detection performance of two Illumina probe-based panels, the Respiratory and the Urinary Pathogen ID panels (RPIP and UPIP), using 99 clini...
Epidemiological Data and Antimicrobial Resistance of Campylobacter spp. in Port...
Duarte, Andreia; Pereira, Luísa; Lemos, Maria-Leonor; Pinto, Miguel; Rodrigues, João Carlos; Matias, Rui; Santos, Andrea; PTCampyNet; Oleastro, MónicaThis study extensively analyzed campylobacteriosis surveillance in Portugal from 2009 to 2021, aiming to investigate demographic shifts, seasonal variations, and antimicrobial resistance (AMR) within Campylobacter isolates. Surveillance network and sentinel laboratory-based system data revealed a substantial under-notification of campylobacteriosis cases, suggesting an underestimated disease burden. Notificatio...
Novas perspetivas sobre o papel e as aplicações dos bacteriófagos de Helicobact...
Ferreira, Rute Vanessa Novais; Oleastro, Mónica; Azeredo, Joana; Figueiredo, Céu; Melo, Luís Daniel RodriguesAs infeções por Helicobacter pylori são caracterizadas por uma elevada incidência global, constituindo uma ameaça significativa à saúde pública. Os tratamentos atuais envolvem a administração de antibióticos e inibidores da bomba de protões. No entanto, esta abordagem terapêutica apresenta uma eficácia limitada, principalmente devido ao aumento da resistência aos antibióticos em todo o mundo. Este cenário salie...
Evaluation of Bile Salts on the Survival and Modulation of Virulence of Aliarco...
Mateus, Cristiana; Maia, Cláudio J.; Domingues, Fernanda; Bücker, Roland; Oleastro, Mónica; Ferreira, SusanaAliarcobacter butzleri is a Gram-negative bacterium associated with infections of the gastrointestinal tract and widely distributed in various environments. For successful infection, A. butzleri should be able to tolerate various stresses during gastrointestinal passage, such as bile. Bile represents an antimicrobial host barrier that acts against external noxious agents and consists of a variety of bile salts....
Colaboração intersetorial na investigação de surtos numa abordagem "Uma Só Saúd...
Manageiro, Vera; Caria, Ana; Furtado, Cristina; SimEx Portuguese Team; Botelho, Ana; Oleastro, Mónica; Gonçalves, Sandra CavacoA colaboração intersetorial é uma componente essencial da abordagem "Uma Só Saúde" (One Health), que reconhece a interligação entre a saúde dos seres humanos, dos animais e do ambiente. O Programa OHEJP (One Health European Joint Programme) desenvolveu um exercício nacional de simulação de surtos de origem alimentar (OHEJP SimEx) com o objetivo de promover a capacitação e interoperabilidade entre os setores da ...
Putative Role of an ABC Efflux System in Aliarcobacter butzleri Resistance and ...
Martins, Inês; Mateus, Cristiana; Domingues, Fernanda; Oleastro, Mónica; Ferreira, SusanaAbstract: Aliarcobacter butzleri is considered a ubiquitous microorganism and emergent pathogen, for which increasing rates of multidrug resistance have been described. In line with this, the present work aimed to evaluate for the first time the contribution of an ABC efflux system, the YbhFSR, in the resistance and virulence of this bacterium. Following the in silico characterization of the YbhFSR transporter,...
Caracterização genotípica e fenotípica de estirpes de Escherichia coli patogéni...
Batista, Rita; Saraiva, Margarida; Lopes, Teresa; Silveira, Leonor; Coelho, Anabela; Furtado, Rosália; Castro, Rita; Correia, Cristina BeloAs aves são potenciais portadoras de microrganismos patogénicos que afetam os seres humanos, e podem ser disseminadoras de perigos no ambiente de produção primária de géneros alimentícios de origem vege- tal e animal. Este trabalho teve como objetivo avaliar a ocorrência, em fezes de aves em liberdade, em Portugal, de três bactérias zoonóticas causadoras de infeções no Homem. Para tal, foi avaliada a presença d...
Editorial for Special Issue Foodborne Pathogens: Infections and Pathogenesis
Oleastro, Mónica; Botelho, AnaFoodborne microorganisms substantially impact food safety and contribute considerably to the public health and economic burden of infectious diseases worldwide [...].