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Microalgal Systems, a Green Solution for Wastewater Conventional Pollutants Rem...

Amaro, HM; Sousa, JF; Salgado, EM; Pires, JCM; Olga C Nunes

The low-efficiency rate of urban wastewater (UWW) treatment generates tons of discharged water with a high concentration of pollutants, pathogens and antibiotic-resistance genes (ARGs). Microalgal systems may be a green alternative to be implemented as a UWW polishing treatment. This study assessed the ability of Chlorella vulgaris and UWW autochthonous microalgal species (AMS) to simultaneously remove PO4-P, a...


Performance of polycarbonate, cellulose nitrate and polyethersulfone filtering ...

Abreu-Silva, J; Ribeirinho-Soares, S; Oliveira-Inocencio, I; Pedrosa, M; Silva, Adrian; Olga C Nunes; Manaia, CM

Demineralized and disinfected waters may have very low microbial loads, requiring that large volumes of water are filtered to recover enough biomass for further analysis. Extended filtration periods, often interrupted by clogging, are a major limiting factor to concentrate samples' microbiota for further examination, besides hindering the work pace. In this study, we investigated the performance of three types ...


A rationale for the high limits of quantification of antibiotic resistance gene...

Fortunato, G.; Ivone Vaz Moreira; Becerra-Castro, C.; Olga C Nunes; Manaia, C.M.


Insights on sulfamethoxazole bio-transformation by environmental Proteobacteria...

Patricia J M Reis; Vera Homem; Arminda Alves; Vitor Vilar; Célia Manaia; Olga C Nunes

Although sulfonamide residues are frequently reported as freshwaters contaminants, information on the ability of native bacteria to modify these synthetic antibiotics is scarce. Our purpose was to investigate the potential of bacteria from different aquatic environments to cleave or transform sulfamethoxazole (SMX) and infer on their ability to reduce the toxicity of this antibiotic. From a collection of about ...


Antibiotic resistance in wastewater treatment plants: Tackling the black box

Manaia, CM; Rocha, J; Scaccia, N; Marano, R; Radu, E; Biancullo, F; Cerqueira, F; Fortunato, G; Iakovides, IC; Zammit, I; Kampouris, I

Wastewater is among the most important reservoirs of antibiotic resistance in urban environments. The abundance of carbon sources and other nutrients, a variety of possible electron acceptors such as oxygen or nitrate, the presence of particles onto which bacteria can adsorb, or a fairly stable pH and temperature are examples of conditions favouring the remarkable diversity of microorganisms in this peculiar ha...


Biodegradation of sulfamethoxazole by a bacterial consortium of Achromobacter d...

Ana Reis; vanarová, M.; Liu, Y.; Lenz, M.; Timm Hettich, T.; Kolvenbach, B.A.; Corvini, P. F.-X.; Olga C Nunes


Complete genome sequence of Achromobacter denitrificans PR1

Reis, AC; Kroll, K; Gomila, M; Kolvenbach, BA; Corvini, PFX; Olga C Nunes

Achromobacter denitrificans strain PR1 was isolated from an enrichment culture able to use sulfamethoxazole as an energy source. Here, we describe the complete genome of this strain sequenced by Illumina MiSeq and Oxford Nanopore MinION. (c) 2017 Reis et al.


Applications of optical DNA mapping in microbiology

Bogas, D; Nyberg, L; Pacheco, R; Azevedo, NF; Beech, JP; Gomila, M; Lalucat, J; Manaia, CM; Olga C Nunes; Tegenfeldt, JO; Westerlund, F

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in ...



Oryzisolibacter propanilivorax gen. nov., sp nov., a propanil-degrading bacterium

Ivone Vaz Moreira; Narciso da Rocha, C; Lopes, AR; Carvalho, G; Lobo da Cunha, A; Whitman, WB; Snauwaert, C; Vandamme, P; Manaia, CM; Olga C Nunes

Strain EPL6(T), a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6(T) was observed to be a member of the family Comamonadaceae, sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601(T) (96.8 %) and Melaminivora ...


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