Encontrados 5 documentos, a visualizar página 1 de 1
Expression in Escherichia coli of cysteine protease inhibitors from cowpea (Vig...
Monteiro Júnior, José Edvar; Valadares, Napoleão Fonseca; Pereira, Humberto D’Muniz; Dyszy, Fábio Henrique; Costa Filho, Antônio José daTwo cysteine proteinase inhibitors from cowpea, VuCys1 and VuCys2, were produced in E. coli ArcticExpress (DE3). The recombinant products strongly inhibited papain and chymopapain as well as the midgut proteases from Callosobruchus maculatus larvae, a bruchid that uses cysteine proteases as major digestive enzymes. Heat treatment at 100 °C for up to 60 min or incubation at various pH values caused little reduct...
Structural and functional evaluation of the palindromic alanine-rich antimicrob...
Migliolo, Ludovico; Felício, Mário Romão; Cardoso, Marlon H.; Silva, Osmar N.; Xavier, Mary-Ann E.; Nolasco, Diego O.; Oliveira, Adeliana Silva deRecently, several peptides have been studied regarding the defence process against pathogenic microorganisms, which are able to act against different targets, with the purpose of developing novel bioactive compounds. The present work focuses on the structural and functional evaluation of the palindromic antimicrobial peptide Pa-MAP2, designed based on the peptide Pa-MAP from Pleuronectes americanus. For a bette...
Trypsin inhibitor from tamarindus indica L. seeds reduces weight gain and food ...
Ribeiro, Joycellane Alline do Nascimento Campos; Serquiz, Alexandre Coellho; Silva, Priscila Fabíola dos SantosOBJECTIVES: Seeds are excellent sources of proteinase inhibitors, some of which may have satietogenic and slimming actions. We evaluated the effect of a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption and cholecystokinin levels in Wistar rats. METHODS: A trypsin inhibitor from Tamarindus was isolated using ammonium sulfate (30-60%) following precipitation with acetone and was ...
Purificação e caracterização de inibidores de tripsina de sementes de Pithecell...
Oliveira, Adeliana Silva deFive Kunitz-type trypsin inhibitors (JB1, JB2, JB3-1, JB3-2 and JB4) were purified from Pithecellobium dumosum seeds, a tree of the sub-family Mimosoideae, by TCA precipitation, affinity chromatography on immobilized trypsin-Sepharose and reverse phase HPLC using Vydac C-18 column. The five inhibitors had Mr between 18 and 20 kDa with a single polypeptide chain as determined by SDS-PAGE with and without reducti...
Purificação e caracterização de inibidores de tripsina de sementes de Pithecell...
Oliveira, Adeliana Silva deOLIVEIRA, A. S de. Purificação e caracterização de inibidores de tripsina de sementes de Pithecellobium dumosun e seus efeitos. 222 f. 2007. Tese (Doutorado em Bioquímica)- Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2007.; Five Kunitz-type trypsin inhibitors (JB1, JB2, JB3-1, JB3-2 and JB4) were purified from Pithecellobium dumosum seeds, a tree of the sub-family Mimosoideae, by TCA precipita...