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Microrganismos colonizadores e seu papel nas infecções de pacientes sob cuidado...
Oliveira, Ana Lúcia Lyrio dePacientes admitidos nas unidades de terapia intensiva são portadores de processos infecciosos de gravidade variável; muitos irão adquirir infecções durante o período de internação. O presente estudo foi conduzido com o objetivo de descrever a participação dos microrganismos colonizadores nos processos infecciosos de pacientes admitidos na terapia intensiva de adultos, em Hospital Universitário de médio porte, n...
Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slid...
Brustoloni, Yvone Maia; Lima, Rosimar Batista; Cunha, Rivaldo Venâncio da; Dorval, Maria Elizabeth; Oshiro, Elisa Teruya; Oliveira, Ana Lúcia Lyrio deThe aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in archived Giemsa-stained bone marrow slides for diagnosis of visceral leishmaniasis (VL), and to compare PCR with conventional diagnostic techniques, like direct microscopy and parasite culture. Specimens of archived Giemsa-stained bone marrow slides from 91 patients with...
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of vi...
Fraga, Thiago Leite; Brustoloni, Yvone Maia; Lima, Rosimar Baptista; Dorval, Maria Elizabeth Cavalheiros; Oshiro, Elisa Teruya; Oliveira, JanainaThe diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peri...
Asymptomatic infection in family contacts of patients with human visceral leish...
Oliveira, Ana Lúcia Lyrio de; Paniago, Anamaria Mello Miranda; Sanches, Marcos Antônio; Dorval, Maria Elizabeth Moraes Cavalheiros; Oshiro, Elisa TeruyaThe Brazilian city of Três Lagoas, Mato Grosso do Sul State, has experienced an urban outbreak of visceral leishmaniasis since 2000. In 2002, due to the increase in the number of cases, 46 families with cases of visceral leishmaniasis were studied to verify the prevalence of asymptomatic infection in household contacts. Indirect immunofluorescence and ELISA showed a 36.4% positive infection rate. There were no ...