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Simultaneous production and recovery in situ of fibrinolytic protease from Muco...

Porto, Ana Lúcia Figueiredo; Sales, Amanda Emmanuelle; Nascimento, Thiago Pajeú; Porto, Camila Souza; Teixeira, J. A.; Campos-Takaki, Galba Maria de


Purification of a fibrinolytic protease from Mucor subtilissimus UCP 1262 by aq...

Nascimento, Thiago Pajeú; Sales, Amanda Emmanuelle; Porto, Camila Souza; Brandão, Romero Marcos Pedrosa; Campos-Takaki, Galba Maria de; Teixeira, J. A.

A fibrinolytic protease from M. subtilissimus UCP 1262 was recovered and partially purified by polyethylene glycol (PEG)/sodium sulfate aqueous two-phase systems (ATPS). The simultaneous influence of PEG molar mass, PEG concentration and sulfate concentration on the enzyme recovery was first investigated using a 23 full factorial design, and the Response Surface Methodology used to identify the optimum conditio...


Production and characterization of new fibrinolytic protease from Mucor subtill...

Nascimento, Thiago Pajeú; Sales, Amanda Emmanuelle; Porto, Camila Souza; Brandão, Romero Marcos Pedrosa; Takaki, G. M. Campos; Teixeira, J. A.

Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources have been extensively studied. The aim of this work was to produce fibrinolytic protease by solid state fermentation using agro industrial substrates. Rhizopus arrhizus var. arrh...


Evaluation the best condition of Fibrinolytic protease production using factori...

Silva, J. M.; Clementino, E. L.; Souza, K. P. S.; Sales, Amanda Emmanuelle; Nascimento, Thiago Pajeú; Teixeira, J. A.; Porto, Tatiana Souza

Fibrinolytic enzymes have the ability to degrade fibrin clots formed for avoiding intravascular thrombosis. In the pharmaceutical industry there is a search for new fibrinolytic agent that reduces the production cost and increasing productivity. The use of microorganism for enzyme production, such as the genus Streptomyces has been reported. Streptomyces is a Gram-positive bacteria, responsible for producing ma...


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