20 documents found, page 1 of 2
Genetic heterogeneity and taxonomic diversity among Gardnerella species
Castro, Joana Isabel Reis; Jefferson, Kimberly K.; Cerca, NunoGardnerella spp. are the most frequent organisms found in bacterial vaginosis (BV). However, colonization by Gardnerella spp. does not always lead to BV. Developments in molecular genetics have shed new light on the diversity of the genus Gardnerella, leading to an emended description of Gardnerella vaginalis and, for the first time, the inclusion of more species of Gardnerella, namely, Gardnerella leopoldii, G...
Innate immune components affect growth and virulence traits of bacterial-vagino...
Castro, Joana; Jefferson, Kimberly K.; Cerca, NunoMucosal surfaces of the female reproductive tract contain a variety of antimicrobial components that provide the first line of defense against bacteria involved in the development of bacterial vaginosis (BV). Microbiological analysis of BV has shown Gardnerella vaginalis to be a prominent species in BV development. However, G. vaginalis colonization does not always lead to BV. Over the last decade, phenotypic a...
Using an in-vitro biofilm model to assess the virulence potential of Bacterial ...
Castro, Joana; Alves, Patrícia Maria; Sousa, Cármen Sofia Vieira; Cereija, Tatiana; França, Ângela Maria Oliveira Sousa; Jefferson, Kimberly K.Gardnerella vaginalis is the most common species found in bacterial vaginosis (BV). However, it is also present in a significant proportion of healthy women and G. vaginalis vaginal colonization does not always lead to BV. In an effort to better understand the differences between G. vaginalis isolated from women with a positive (BV) versus a negative (non-BV) diagnosis of BV, we compared the virulence potential...
Functional analysis of virulence potential from Gardnerella vaginalis and other...
Castro, J.; Machado, António; Alves, Patrícia Maria; Sousa, Cármen; Cereija, Tatiana Barros Reis; França, Ângela; Jefferson, Kimberly K.; Cerca, NunoIn the past half century, bacterial vaginosis (BV) has been a controversial topic in medical microbiology, and despite the wealth of information on this topic, the etiological agent has not yet been definitively identified [1]. The first advances on BV pointed Gardnerella vaginalis as the infectious causative agent of BV [2] but soon after it was found that G. vaginalis was also present in healthy women [3]. Ad...
Interactions between Lactobacillus crispatus and bacterial vaginosis (BV)-Assoc...
Machado, António; Jefferson, Kimberly K.; Cerca, NunoCertain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (BV), with Gardnerella vaginalis being the most common. However, the exact role of G. vaginalis in BV has not yet been determined. The main goal of this study was to test the hypothesis that G. vaginalis is an early colonizer, paving the way for intermediate (e.g., Fusobacterium nucleatum) and late colonizers (e...
Reciprocal interference between Lactobacillus spp. and Gardnerella vaginalis on...
Castro, J.; Henriques, Ana Filipa Frutuoso Mendes; Machado, António; Henriques, Mariana; Jefferson, Kimberly K.; Cerca, NunoBacterial vaginosis (BV) is the most common vaginal disorder in women of child-bearing age. It is widely accepted that the microbial switch from normal microflora to the flora commonly associated with BV is characterized by a decrease in vaginal colonization by specific Lactobacillus species together with an increase of G. vaginalis and other anaerobes. However, the order of events leading to the development of...
Quantitative analysis of initial adhesion of bacterial vaginosis-associated ana...
Machado, António; Salgueiro, Débora; Harwich, Michael; Jefferson, Kimberly K.; Cerca, NunoBacterial vaginosis is the leading vaginal disorder but the transition from health to this dysbiotic condition remains poorly characterized. Our goal was to quantify the ability of BV-associated anaerobes to adhere to epithelial cells in the presence of lactobacilli. Gardnerella vaginalis outcompeted Lactobacillus crispatus and Lactobacillus iners actually enhanced its adherence.; We want to generously thank to...
Quantitative analysis of initial adhesion of bacterial vaginosis anaerobes in M...
Machado, António; Salgueiro, Débora; Harwich, Michael; Jefferson, Kimberly K.; Cerca, NunoBacterial vaginosis (BV) is the most common vaginal disorder in women of reproductive age [1]. Despite decades of research, the etiology of BV still remains elusive. It is well established that adhesion to host cells or tissues is a necessary early step in the establishment of infection [2]. Since it is often considered a polymicrobial condition, it has been proposed that some bacteria have a preponderant role ...
Competitive initial adhesion between Lactobacillus spp. and Gardnerella vaginal...
Castro, J.; Machado, António; Henriques, Ana Filipa Frutuoso Mendes; Henriques, Mariana; Jefferson, Kimberly K.; Cerca, NunoBacterial vaginosis (BV) is a common disorder in women of reproductive age. BV is characterized by the replacement of vaginal lactobacilli, such as Lactobacillus crispatus, by predominantly anaerobic microorganisms. However, Lactobacillus iners is frequently found in the BV. Gardnerella vaginalis, commonly associated with BV, can also be present in 50-95% of BV patients and in 20-30% of healthy women. The capac...
Social interaction by BV anaerobes in initial adhesion and biofilm assays
Machado, António; Salgueiro, Débora; Rodrigues, L. R.; Cerca, Nuno; Jefferson, Kimberly K.Bacterial vaginosis (BV) is the most common vaginal disorder of women of reproductive age. It is commonly accepted that the microbial switch from normal to BV state is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase in the number of Gardnerella vaginalis and others anaerobes. G. vaginalis can be found associated with normal vaginal epithelium but recent fin...