108 documents found, page 1 of 11
Optical immunosensor panel using quantum dot-antibody conjugates for highly sen...
Oliveira, Daniela; Oliveira, Pedro; Xu, Ana; Rodrigues, Eduarda; Guerreiro, Susana G.; Castro, Rafael C.; Ribeiro, David S. M.; Santos, João L. M.This study introduces a fluorescent immunosensor colour panel with antibody-conjugated quantum dots for detecting CA19-9 in human serum. The immunosensors presented decreasing fluorescence with increasing CA19-9 concentrations, with a linear detection range from 0.01 to 501.87 U mL−1 and detection limits of 1.66 × 10⁻⁴, 2.71 × 10⁻⁴, and 5.45 × 10⁻⁴ U mL−1 for the green-, orange-, and red-emitting conjugates in ...
Anterior fontanelle closure time in 684 healthy Portuguese children
Costa, Ricardo Craveiro; Rosmaninho Salgado, Joana; Oliveira, Daniela; Mendes, Francisca; Silva, Alexandre; Salgado, ManuelIntroduction and Aim: The timing of anterior fontanelle (AF) closure may be a useful marker for early disease detection. However, the few published studies on this topic have reported significant differences in the normal age ranges of AF closure between countries. The aim of this study was to report AF closure time in the Portuguese population during the last two decades. Methods: This was a 21-year retrospect...
Protective properties of the edible halophyte Carpobrotus edulis (L.) N.E.Br. t...
Oliveira, Daniela; Hayrapetyan, Ruzanna; Dias, Maria Inês; Barros, Lillian; Séverin, Isabelle; Custódio, Luísa; Chagnon, Marie-ChristineCarpobrotus edulis (L.) N.E.Br. is an edible halophyte native to South Africa, where it has been traditionally consumed as food, revealing its nutritional value, and used in the treatment of human diseases probably due to the accumulation of bioactive compounds such as polyphenols. These compounds have potential to display valuable biological activities that could bring several benefits to the human being, incl...
SERS biosensor with plastic antibodies for detection of a cancer biomarker protein
Oliveira, Daniela; Carneiro. Mariana C. C. G.; Moreira, Felismina T. C.Surface-enhanced Raman scattering (SERS) is a powerful method for detecting breast cancer-specific biomarkers due to its extraordinary enhancement effects obtained by localized surface plasmon resonance (LSPR) in metallic nanostructures at hotspots. In this research, gold nanostars (AuNSs) were used as SERS probes to detect a cancer biomarker at very low concentrations. To this end, we combined molecularly impr...
Development of an immunosensor based on quantum dots with different emissions f...
Oliveira, Daniela; Piloto, Ana Margarida L.(Introduction) Cancer is a global health challenge, where early detection is crucial to increasing survival rates [1]. Non-invasive, rapid, and affordable diagnostic methods are urgently needed. Immunosensors emerge as promising alternatives to traditional methods such as ELISA, allowing point-of-care (PoC) analyses [2]. This study introduces a fluorescent probe using cadmium telluride quantum dots (CdTe@MPA QD...
An electrochemically synthesized molecularly imprinted polymer for highly selec...
Oliveira, Daniela; Barcelay, Yonny Romaguera; Moreira, Felismina T. C.In this study, a molecularly imprinted polymer film (MIP) was prepared on the surface of a disposable carbon screen-printed electrode (C-SPE) using (3-acrylamidopropyl)trimethylammonium chloride (AMPTMA) as a functional monomer and the cancer biomarker carbohydrate antigen 15-3 (CA 15-3) as a template. The MIP was synthesized by in situ electropolymerization (ELP) of the AMPTMA monomer in the presence of the CA...
Ratiometric optical visual immunosensor based on quantum dots for CA 19-9 prote...
Oliveira, Daniela; Piloto, Ana Margarida L.Cancer is a global health challenge, where early detection is crucial to increasing survival rates. Non-invasive, rapid, and affordable diagnostic methods are urgently needed. Immunosensors emerge as promising alternatives to traditional methods such as ELISA, allowing point-of-care (PoC) analyses. This study aims to develop a highly sensitive and selective optical immunosensor for detecting CA 19-9. The result...
An electrochemically synthesized molecularly imprinted polymer for highly selec...
Oliveira, Daniela; Romaguera Barcelay, Yonny; Moreira, FelisminaIn this study, a molecularly imprinted polymer film (MIP) was prepared on the surface of a disposable carbon screen-printed electrode (C-SPE) using (3-acrylamidopropyl)trimethylammonium chloride (AMPTMA) as a functional monomer and the cancer biomarker carbohydrate antigen 15-3 (CA 15-3) as a template. The MIP was synthesized by in situ electropolymerization (ELP) of the AMPTMA monomer in the presence of the CA...
Molecularly imprinting for ca 15-3 protein detection: a promising biosensor for...
Oliveira, Daniela; Barcelay, Yonny Romaguera; Moreira, Felismina(Introduction) Cancer is a public health problem worldwide. Early detection is crucial to improve survival rates [1]. Non-invasive, fast rapid, and low-cost diagnostic methods are urgently needed. Biosensors are a promising alternative to traditional methods such as ELISA, enabling point-ofcare (PoC) analyses. These require biorecognition elements with high selectivity and stability to meet current requirements...
Enhanced detection with prussian blue-based nanocubes: a novel electrochemical ...
Santos, Andréa dos; Oliveira, Daniela; Martins, Gabriela; Moreira, Felismina T.C.(Introduction) Early diagnosis of biomarkers is crucial for optimizing treatments and increasing survival rates. Direct and cost-effective assays are important for point-of-care (PoC) testing. Bovine serum albumin (BSA) monitoring indicates liver and kidney function and aids in the assessment of diseases such as cirrhosis and chronic kidney disease. An innovative electrochemical biosensor was developed in which...