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Adult Acne: Prevalence and Portrayal in Primary Healthcare Patients, in the Gre...

Semedo, Diogo; Ladeiro, Filipa; Ruivo, Mariana; d'Oliveira, Carlos; de Sousa, Filipa; Gayo, Mariana; Lima, Clara; Magalhães, Frederico; Brandão, Rui

Introduction: Though typically found in adolescents, acne frequently affects the adult population. In Portugal, no representative studies about acne prevalence in greater populational areas are known. This study had the following objectives: calculate acne prevalence in primary healthcare patients; its characteristics, including risk factors for onset and worsening; collect information concerning selfmedication...

Date: 2016   |   Origin: Acta Médica Portuguesa

Adult acne: prevalence and portrayal in primary healthcare patients, in the gre...

Semedo, Diogo; Ladeiro, Filipa; Ruivo, Mariana; Oliveira, Carlos; Sousa, Filipa de; Gayo, Mariana; Lima, Clara; Magalhães, Frederico; Brandão, Rui

Introduction: Though typically found in adolescents, acne frequently affects the adult population. In Portugal, no representative studies about acne prevalence in greater populational areas are known. This study had the following objectives: calculate acne prevalence in primary healthcare patients; its characteristics, including risk factors for onset and worsening; collect information concerning self-medicatio...


High-level expression of Aspergillus niger β-galactosidase in Ashbya gossypii

Magalhães, Frederico; Aguiar, Tatiana Quinta; Oliveira, Carla Cristina Marques de; Domingues, Lucília

Ashbya gossypii has been recently considered as a host for the expression of recombinant proteins. The production levels achieved thus far were similar to those obtained with Saccharomyces cerevisiae for the same proteins. Here, the β-galactosidase from Aspergillus niger was successfully expressed and secreted by A. gossypii from 2-micron plasmids carrying the native signal sequence at higher levels than those ...


Molecular and functional characterization of an invertase secreted by Ashbya go...

Aguiar, Tatiana Quinta; Dinis, Cláudia; Magalhães, Frederico; Oliveira, Carla Cristina Marques de; Wiebe, Marilyn G.; Penttilä, Merja

The repertoire of hydrolytic enzymes natively secreted by the filamentous fungus Ashbya (Eremothecium) gossypii has been poorly explored. Here, an invertase secreted by this flavinogenic fungus was for the first time molecularly and functionally characterized. Invertase activity was detected in A. gossypii culture supernatants and cell-associated fractions. Extracellular invertase migrated in a native polyacryl...


Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii

Ribeiro, Orquídea; Magalhães, Frederico; Aguiar, Tatiana Quinta; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, Lucília

To improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endoglucanase I (EGI), native α-amylase and/or native β-glucosidase. One mutant, S436, presented 1.4- to 2-fold increases i...


High expression levels of Aspergillus niger β -galactosidase in Ashbya gossypii

Magalhães, Frederico; Dinis, Cláudia; Aguiar, Tatiana Quinta; Oliveira, Carla Cristina Marques de; Domingues, Lucília

Ashbya gossypii is a filamentous Saccharomycete that has been recently considered as a host for the expression of recombinant proteins. However, the expression levels obtained were low, even though similar to those observed in Saccharomyces cerevisiae for the same proteins. Here, to further assess the potential of this fungus as a recombinant protein producer, the -galactosidase from Aspergillus niger was succe...


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