8 documents found, page 1 of 1
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Cro...
Kerkis, Alexandre; Kerkis, Irina; Rádis-Baptista, Gandhi; Oliveira, Eduardo B.; Vianna-Morgante, Angela M.; Pereira, Lygia V.; Yamane, TetsuoHerein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed...
Cytotoxic effects of crotamine are mediated through lysosomal membrane permeabi...
Hayashi, Mirian A.F.; Nascimento, Fábio D.; Kerkis, Alexandre; Oliveira, Vitor; Oliveira, Eduardo B.; Pereira, Alexandre; Rádis-Baptista, GandhiCrotamine, one of the main toxic components of Crotalus durissus terrificus venom, is a small non-enzymatic basic polypeptide, which causes hind limb paralysis and necrosis of muscle cells. It is well-known that several toxins penetrate into the cytosol through endocytosis, although in many cases the mechanism by which this occurs has not been fully investigated. Recently, using low concentrations of crotamine,...
Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue
Santos, Carlos F.; Morandini, Ana C.; Dionisio, Thiago J.; Faria, Flavio A.; Lima, Marta C.; Figueiredo, Caio M.; Colombini-Ishikiriama, Bella L.Made available in DSpace on 2018-11-26T17:55:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-08-05; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); University of Sao Paulo [USP]; The initiation or progression of periodontitis might involve a local re...
Design of bio-based supramolecular structures through self-assembly of α-lactal...
Monteiro, Adenilson A.; Monteiro, Márcia R.; Pereira, Ricardo N.; Diniz, Renata; Costa, Angélica R.; Malcata, F. Xavier; Teixeira, José A.Bovine α-lactalbumin (α-La) and lysozyme (Lys), two globular proteins with highly homologous tertiary structures and opposite isoelectric points, were used to produce bio-based supramolecular structures under various pH values (3, 7 and 11), temperatures (25, 50 and 75 °C) and times (15, 25 and 35 min) of heating. Isothermal titration calorimetry experiments showed protein interactions and demonstrated that str...
Structure-function studies of BPP-BrachyNH 2 and synthetic analogues thereof wi...
Arcanjo, Daniel D.R.; Vasconcelos, Andreanne G.; Nascimento, Lucas A.; Mafud, Ana Carolina; Plácido, Alexandra; Alves, Michel M.M.The vasoactive proline-rich oligopeptide termed BPP-BrachyNH2 (H-WPPPKVSP-NH2) induces in vitro inhibitory activity of angiotensin I-converting enzyme (ACE) in rat blood serum. In the present study, the removal of N-terminal tryptophan or C-terminal proline from BPP-BrachyNH2 was investigated in order to predict which structural components are important or required for interaction with ACE. Furthermore, the tox...
Design of bio-based supramolecular structures through self-assembly of α-lactal...
Monteiro, Adenilson A.; Monteiro, Marcia R.; Pereira, Ricardo N.; Diniz, Renata; Costa, Angélica R.; Malcata, F. Xavier; Teixeira, J. A.Bovine α-lactalbumin (α-La) and lysozyme (Lys), two globular proteins with highly homologous tertiary structures and opposite isoelectric points, were used to produce bio-based supramolecular structures under various pH values (3, 7 and 11), temperatures (25, 50 and 75 °C) and times (15, 25 and 35 min) of heating. Isothermal titration calorimetry experiments showed protein interactions and demonstrated that str...
Design of nanostructures, obtained from assembling of α-lactalbumin and lysozym...
Monteiro, Adenilson A.; Monteiro, Márcia R.; Ramos, Óscar L.; Pereira, Ricardo; Diniz, Renata; Costa, Angélica R.; Malcata, F. X.; Teixeira, J. A.Protein assembly into supramolecular structures (e.g. aggregates, fibrils and nanotubes) is a widespread phenomenon in biological sciences. Nowadays, it is well documented that the amphiphilic properties of proteins is a driving force to their self-assembling into innovative micro- and nanostructures of high interest in the food and pharmaceutical fields. Formation of such structures is strongly dependent on ph...
Nanoencapsulation of quercetin into bio-based nanostructures obtained from asse...
Monteiro, Adenilson A.; Monteiro, Marcia R.; Ramos, Óscar L.; Pereira, Ricardo; Malcata, F. X.; Teixeira, J. A.; Teixeira, Álvaro V.Nanotechnology possesses an intrinsic potential to produce new food ingredients and innovative products, with considerable benefits to human health. This can be attained via development of innovative structures for application in functional foods. In recent years, consumption of foods providing health benefits has risen chiefly as a result of significant investments from the food industry and widening consumer ...