8 documents found, page 1 of 1

Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosts of...

Made available in DSpace on 2019-09-12T16:53:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2008; A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n = 23) and chronic (n = 30) schistosomiasis, with other parasitoses (n = 39) or without parasitic infections (n = 10...

Schistosomiasis mansoni: follow-up of control program based on parasitologic an...

Made available in DSpace on 2019-09-11T20:58:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2003; A field survey on schistosomiais was carried out in 1998, in the municipality of Pedro de Toledo, a low endemic area in the state of São Paulo, Brazil. According to the parasitologic Kato-Katz method, the prevalence rate was 1.6%, with an infection intensity of 40.9 eggs per gram of stool. By the immunofluore...

Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schis...

Made available in DSpace on 2019-09-11T20:58:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2005; IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schistosoma mansoni adult worm antigen (AWA) that is soluble in trichloroacetic acid (AWA-TCA). This method was applied to three groups of individuals with different clinical and epidemiological characteristics...

Application of synthetic peptides in development of a serologic method for labo...

Made available in DSpace on 2019-09-11T20:58:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2006; The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventiona...

Evaluation of three recombinant proteins for the development of ELISA and immun...

BACKGROUND Visceral Leishmaniasis (VL) is an infectious disease that is a significant cause of death among infants aged under 1 year and the elderly in Brazil. Serodiagnosis is a mainstay of VL elimination programs; however, it has significant limitations due to low accuracy. OBJECTIVE This study aimed to evaluate three recombinant Leishmania infantum proteins (rFc, rC9, and rA2) selected from previous proteomi...

Multi-centric prospective evaluation of rk39 rapid test and direct agglutinatio...

Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. L...

Characterization of a clone from an adult worm cDNA library selected with anti-...

Considering the scarcity of defined antigens, actually useful and reliable for use in the field studies, we propose an alternative method for selection of cDNA clones with potential use in the diagnosis of schistosomiasis. Human antibodies specific to a protein fraction of 31/32 kDa (Sm31/32), dissociated from immune complexes, are used for screening of clones from an adult worm cDNA library. Partial sequencing...

Development of a serologic method for laboratory diagnosis of the schistosomias...