The aim of this study was to histologically evaluate the testes of prepubertal dogs vitrified with different cryoprotectant associations. For this purpose, 10 testicles from prepubertal dogs were used, which were fragmented into samples of 1 to 2 mm3 and vitrified in different cryoprotectant associations: dimethylsulfoxide (DMSO)/ethylene glycol (EG), EG/glycerol(GLY) and DMSO/GLY. After a period of storage, th...