This report describes the genomes of Klebsiella pneumoniae phages SF_KL2 and SF_KL25, which infect the encapsulated multidrug-resistant K. pneumoniae known to cause nosocomial infections. SF_KL2 and SF_KL25 belong to the genus Webervirus (siphovirus morphotype) and have genomes of 48,737 and 49,170 bp, respectively. The phages were isolated from wastewater in northern Portugal.
Bacteriophages (phages) are the most predominant and genetically diverse biological entities on Earth. Phages are viruses that infect bacteria and encode numerous proteins with potential biotechnological application. However, most phage-encoded proteins remain functionally uncharacterized. Depolymerases (DPOs) in particular, enzymes that degrade external polysaccharide structures, have garnered increasing inter...
[Excerpt] Erwinia amylovora, a major pathogenic bacterium affecting pome fruit plants, causes fire blight, a devastating disease of pears and apples (1 ]. lnfection typically begins in flowers and leads to the blackening of leaves, stems, and immature fruits (2). E. amy/ovora is widespread in North and Central America and Europe and spreads through insects, rain, wind, infected propagating material, and non-dis...
Pseudomonas syringae pv. actinidiae (Psa) is the causative agent of bacterial canker in kiwifruit (Actinidia spp.). Psa biovar 3 is the most prevalent and virulent, causing frequent and severe outbreaks worldwide. While current treatments have low efficacy, bacteriophages emerge as possible environmentally safe alternative biocontrol agents. In this study, bacteriophage Brt\_Psa3 was isolated from the soil of a...
O fogo bacteriano, causado pela bactéria Erwinia amylovora, é uma doença altamente destrutiva que afecta membros da família Rosaceae, com impactos particularmente graves em pomares comerciais de macieiras e pereiras em todo o mundo. Os actuais métodos de controlo baseados em produtos químicos têm-se revelado largamente ineficazes e prejudiciais para o ambiente, sublinhando a necessidade urgente de alternativas ...
We report the genome of Erwinia amylovora phage Omen, isolated from a Portuguese orchard. Omen has a genome size of 85,304 bp, belongs to the genus Kolesnikvirus (myovirus morphotype), and shares over 80% nucleotide identity with various Erwinia phage genomes.
Klebsiella spp. are important pathogens of humans and companion animals such as cats and dogs, capable of causing severe life-threatening diseases. The aim of this study was to characterize the molecular and phenotypic properties of Klebsiella pneumoniae and Klebsiella oxytoca isolated from ill companion animals by whole genome sequencing, followed by in vitro assessment of biofilm formation and in vivo pathoge...
Purpose: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important nosocomial pathogen. The capsular type (K-type) is considered a major virulence factor, contributing to the evasion of host defenses. The global spread and dissemination dynamics between K-types, sequence types (ST), antibiotic resistance genes, and virulence factors remain largely unknown in Portugal. Methods: A collection of 96 CRAB ...