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Genome sequences of Klebsiella pneumoniae bacteriophages SF_KL2 and SF_KL25

Lorenz, Sophia; Abreu, Gabriel Ferreira; Oliveira, Hugo Alexandre Mendes

This report describes the genomes of Klebsiella pneumoniae phages SF_KL2 and SF_KL25, which infect the encapsulated multidrug-resistant K. pneumoniae known to cause nosocomial infections. SF_KL2 and SF_KL25 belong to the genus Webervirus (siphovirus morphotype) and have genomes of 48,737 and 49,170 bp, respectively. The phages were isolated from wastewater in northern Portugal.


PhageDPO: A machine-learning based computational framework for identifying phag...

Vieira, Maria Fernanda; Duarte, José; Domingues, Rita; Oliveira, Hugo Alexandre Mendes; Dias, Oscar

Bacteriophages (phages) are the most predominant and genetically diverse biological entities on Earth. Phages are viruses that infect bacteria and encode numerous proteins with potential biotechnological application. However, most phage-encoded proteins remain functionally uncharacterized. Depolymerases (DPOs) in particular, enzymes that degrade external polysaccharide structures, have garnered increasing inter...


Evaluation of antimicrobial efficacy of current methods to control fire blight ...

Araújo, Daniela Eira; Castro, Joana Isabel Reis; Vindeirinho, João; McGuire, Daniel; Cruz, Leonor; Almeida, Carina Manuela Fernandes

[Excerpt] Erwinia amylovora, a major pathogenic bacterium affecting pome fruit plants, causes fire blight, a devastating disease of pears and apples (1 ]. lnfection typically begins in flowers and leads to the blackening of leaves, stems, and immature fruits (2). E. amy/ovora is widespread in North and Central America and Europe and spreads through insects, rain, wind, infected propagating material, and non-dis...


Lytic properties and genomic analysis of bacteriophage Brt_Psa3, targeting Pseu...

Gimranov, Emil; Oliveira, Hugo Alexandre Mendes; Santos, Conceição; Moura, Luísa; Azeredo, Joana

Pseudomonas syringae pv. actinidiae (Psa) is the causative agent of bacterial canker in kiwifruit (Actinidia spp.). Psa biovar 3 is the most prevalent and virulent, causing frequent and severe outbreaks worldwide. While current treatments have low efficacy, bacteriophages emerge as possible environmentally safe alternative biocontrol agents. In this study, bacteriophage Brt\_Psa3 was isolated from the soil of a...


Bacteriófagos: uma nova estratégia para o biocontrolo de doenças bacterianas de...

Abreu, Gabriel Ferreira; Costa Garcia, Edgar; Mortinho, Diana; Soares, Ana; Carvalho, Luís; Oliveira, Hugo Alexandre Mendes


Isolamento e caraterização de novos bacteriófagos como agentes de biocontrolo c...

Abreu, Gabriel Ferreira; Costa Garcia, Edgar; Rodrigues, Carlos; Oliveira, Hugo Alexandre Mendes

O fogo bacteriano, causado pela bactéria Erwinia amylovora, é uma doença altamente destrutiva que afecta membros da família Rosaceae, com impactos particularmente graves em pomares comerciais de macieiras e pereiras em todo o mundo. Os actuais métodos de controlo baseados em produtos químicos têm-se revelado largamente ineficazes e prejudiciais para o ambiente, sublinhando a necessidade urgente de alternativas ...


Genome sequence of Erwinia amylovora bacteriophage Omen

Abreu, Gabriel Ferreira; Costa Garcia, Edgar; Oliveira, Ana; Oliveira, Hugo Alexandre Mendes

We report the genome of Erwinia amylovora phage Omen, isolated from a Portuguese orchard. Omen has a genome size of 85,304 bp, belongs to the genus Kolesnikvirus (myovirus morphotype), and shares over 80% nucleotide identity with various Erwinia phage genomes.


O fogo bacteriano na produção de peras e maçãs: caracterização da situação naci...

Costa Garcia, Edgar; Abreu, Gabriel Ferreira; Martins, Carmo; Marinho, Rita; Pereira, Cláudia; Carvalho, Luís; Almeida, Carina; Cruz, Leonor


Molecular characterization and virulence profile of Klebsiella pneumoniae and K...

Castro, Joana Isabel Reis; Oliveira, Ricardo; Fernandes, Liliana Sousa; Carvalho, Isabel; Oliveira, Hugo Alexandre Mendes; Brinks, Erik; Cho, Gyu-Sung

Klebsiella spp. are important pathogens of humans and companion animals such as cats and dogs, capable of causing severe life-threatening diseases. The aim of this study was to characterize the molecular and phenotypic properties of Klebsiella pneumoniae and Klebsiella oxytoca isolated from ill companion animals by whole genome sequencing, followed by in vitro assessment of biofilm formation and in vivo pathoge...


Molecular detection of carbapenemases in Acinetobacter baumannii strains of Por...

Domingues, Rita; Oliveira, Ricardo; Silva, Sónia Carina; Araújo, Daniela; Almeida, Carina; Cho, Gyu-Sung; Franz, Charles M. A. P.; Saavedra, Maria José

Purpose: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important nosocomial pathogen. The capsular type (K-type) is considered a major virulence factor, contributing to the evasion of host defenses. The global spread and dissemination dynamics between K-types, sequence types (ST), antibiotic resistance genes, and virulence factors remain largely unknown in Portugal. Methods: A collection of 96 CRAB ...


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