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Strategies to optimize anaerobic co-digestion of organic wastes

Oliveira, João Vítor

Atualmente, os processos de digestão anaeróbica apresentam-se como uma tecnologia madura com várias aplicações à escala mundial, incluindo tecnologias complexas e diversificadas de tratamento de resíduos e produção de energia. No entanto, algumas dessas ainda podem ser otimizadas. A codigestão anaeróbia, que consiste na mistura de dois ou mais substratos com características complementares, equilibrando os parâm...


Modelling anaerobic digestion of sewage sludge: mechanistic models vs machine l...

Duarte, Maria Salomé Lira; Martins, Gilberto; Oliveira, João Vítor; Oliveira, P.; Silva, Sérgio Alves; Novais, Paulo; Pereira, M. A.; Alves, M. M.

Anaerobic digestion processes are one of the technologies most used by wastewater treatment plants (WWTPs) to stabilize and decrease the organic content of sludge. This process decreases the costs of disposal while increasing the energetic efficiency of WWTPs. In order to optimize this process, three model approaches were implemented. First, we calibrated and validated the anaerobic digestion model no.1 (ADM1) ...


Effect of endogenous methane production: a step forward in the validation of bi...

Oliveira, João Vítor; Costa, J. C.; Cavaleiro, A. J.; Pereira, M. A.; Alves, M. M.

This work evaluates the influence of the inoculum type, the pre-consumption of the residual substrate and the ratio of blanks’ headspace volume to working volume (Hv Wv−1, 0.6 to 10) on Biochemical Methane Potential (BMP) measurements when methane is monitored by gas chromatography. Different inocula were tested: digested sewage sludge—DSS, granular sludge—GS and fresh dairy manure—DM. Microcrystalline cellulos...


Bacterial cellulose production: valorization of wastewater and life cycle asses...

Dourado, Fernando; Forte, Ana Cristina Figueiras; Mota, André; Neto, Belmira; Ferreira, Eugénio C.; Silva, Francisco Almeida Garrett Soares

Low-cost substrates, most from agro-industrial wastes, have increasingly been exploited as nutrient sources for the fermentation of bacterial cellulose (BC), an appealing approach from an economical and environmental point of view. However, these wastes carry a very high organic load, which, while advantageous for the fermentation, generate high organic load wastewaters as well, which require proper treatment b...


Volatile fatty acids (VFA) production from wastewaters with high salinity-influ...

Duarte, Maria Salomé Lira; Oliveira, João Vítor; Pereira, Carla Daniela Silva; Carvalho, Miguel Saraiva; Mesquita, D. P.; Alves, M. M.

The hydrocarbon-based economy is moving at a large pace to a decarbonized sustainable bioeconomy based on biorefining all types of secondary carbohydrate-based raw materials. In this work, 50 g L1 in COD of a mixture of food waste, brine and wastewater derived from a biodiesel production facility were used to produce organic acids, important building-blocks for a biobased industry. High salinity (1218 g L1), di...


Study and valorisation of wastewaters generated in the production of bacterial ...

Soares da Silva, F. A. G.; Oliveira, João Vítor; Felgueiras, Catarina; Dourado, Fernando; Gama, F. M.; Alves, M. M.

Two culture media were tested for the production of bacterial nanocellulose (BNC) under static culture fermentation, one containing molasses (Mol-HS), the other molasses and corn steep liquor (Mol-CSL), as a source of carbon and nitrogen, respectively. These are low-cost nutrients widely available, which provide very good BNC productivities. However, the use of these substrates generates wastewaters with high o...


Aerobic and facultative bacteria: working horses at the service of anaerobic di...

Duarte, Maria Salomé Lira; Oliveira, João Vítor; Magalhães, Carla Isabel Pereira; Salvador, Andreia Filipa Ferreira; Carvalho, Ana Rita Castro

It is clear that aerobic and facultative anaerobic bacteria have an important role in the first steps of the anaerobic digestion (AD) process, especially when complex organic compounds are degraded. However, their diversity, abundance and function, related to the fine control of process variables such as pH and ORP, and the potential establishment of networks with methanogens and acetogens are far from being fu...


Biochemical methane potential of brewery by-products

Oliveira, João Vítor; Alves, M. M.; Costa, J. C.

Beer production generates by-products with high energy potential, namely trub (Tr, dead yeast from the fermentation) and spent grain (SG, smashed barley grains). This work investigates the biochemical methane potential (BMP, volume of methane produced per volatile solids of substrateL kg1) of these by-products, performing batch anaerobic biodegradability assays. Single substrates were evaluated as well as a mix...


Neutral lipid production from hydrocarbon-contaminated cork sorbents using Rhod...

Castro, Rita; Guimarães, Maura; Oliveira, João Vítor; Pereira, M. A.


Production of added value bacterial lipids through valorisation of hydrocarbon-...

Castro, A. R.; Guimarães, Maura Francisca Silva; Oliveira, João Vítor; Pereira, M. Alcina

This work demonstrates that cork used as oil-spill sorbents, contaminated with liquid hydrocarbons, herein demonstrated with hexadecane, can be biologically treated by Rhodococcus opacus B4 with concomitant lipids production. R. opacus B4 consumed up to 96% of hexadecane (C16) impregnated in natural and regranulated cork sorbents after 48 h incubation, producing 0.59 ± 0.06 g of triacylglycerol (TAG) g 1 of C16...


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