4 documents found, page 1 of 1
A trypsin inhibitor purified from Cassia leiandra seeds has insecticidal activi...
Dias, Lucas P.; Oliveira, Jose T. A.; Rocha-Bezerra, Lady C. B.; Sousa, Daniele O. B.; Costa, Helen P. S.; Araújo, Nadine M. S.; Carvalho, Ana F. U.A trypsin inhibitor from Cassia leiandra seeds, named ClTI, was purified, characterized, and its insecticidal activity against Aedes aegypti evaluated. ClTI was purified by DEAE-Cellulose and trypsin-Sepharose 4B chromatography, with a 15.5-fold purification and 2.4% yield. ClTI is composed of a 19,484 Da polypeptide chain as revealed by mass spectrometry, it is not a glycoprotein, its amino acid sequence is si...
Food safety assessment of an antifungal protein from Moringa oleifera seeds in ...
Pinto, Clidia E. M.; Farias, Davi F.; Carvalho, Ana F. U; Oliveira, Jose T. A.; Pereira, Mirella L.; Grangeiro, Thalles B.; Freire, Jose E. C.Mo-CBP3 is an antifungal protein produced by Moringa oleifera which has been investigated as potential candidate for developing transgenic crops. Before the use of novel proteins, food safety tests must be conducted. This work represents an early food safety assessment of Mo-CBP3, using the two-tiered approach proposed by ILSI. The history of safe use, mode of action and results for amino acid sequence homology...
A novel peroxidase purified from Marsdenia megalantha latex inhibits phytopatho...
Oliveira, Henrique P.; Silva, Rodolpho G. G; Oliveira, Jose T. A.; Sousa, Daniele O. B.; Pereira, Mirella L.; Souza, Pedro F. N.; Soares, Arlete A.An antifungal class III peroxidase was purified from Marsdenia megalantha latex (named Mo-POX) using DEAE-cellulose and gel filtration chromatography on a Superose 12 HR 10/30 column. Mm-POX has an apparent molecular mass of 67.0 kDa and a pI of 5.2, shares identity with other peroxidases, and fol lows Michaelis-Menten kinetics. It has a high affinity for guaiacol and hydrogen peroxide. The pH and temperature o...
A chitin-binding protein purified from Moringa oleifera seeds presents anticand...
S. Neto, João X.; Pereira, Mirella L.; Oliveira, Jose T. A.; Bezerra, Lady C. B. Rocha; Lopes, Tiago D. P.; Costa, Helen P. S.; Sousa, Daniele O. B.Candida species are opportunistic pathogens that infect immunocompromised and/or immunosuppressed patients, particularly in hospital facilities, that besides representing a significant threat to health increase the risk of mortality. Apart from echinocandins and triazoles, which are well tolerated, most of the antifungal drugs used for candidiasis treatment can cause side effects and lead to the development of ...