12 documents found, page 1 of 2
Screening of perfused combinatorial 3D microenvironments for cell culture
Lopes, Diana; Fernandes, C.; Miguel Nobrega, J.; Patricio, Sonia G.; Oliveira, Mariana Braga; Mano, J. F.Biomaterials combining biochemical and biophysical cues to establish close-to-extracellular matrix (ECM) models have been explored for cell expansion and differentiation purposes. Multivariate arrays are used as material-saving and rapid-to-analyze platforms, which enable selecting hit-spotted formulations targeting specific cellular responses. However, these systems often lack the ability to emulate dynamic me...
The effects of platelet lysate patches on the activity of tendon-derived cells
Almeida, Raquel Carvalho Ferreira Costa; Franco, Albina Ribeiro; Pesqueira, Tamagno; Oliveira, Mariana Braga; Babo, Pedro S.; Leonor, I. B.; Mano, J. F.Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeuti...
The influence of surface modified poly(L-lactic acid) films on the differentiat...
Correia, Clara R.; Gaifem, Joana; Oliveira, Mariana Braga; Silvestre, Ricardo Jorge Leal; Mano, J. F.Macrophages play a crucial role in the biological performance of biomaterials, as key factors in defining the optimal inflammation-healing balance towards tissue regeneration and implant integration. Here, we investigate how different surface modifications performed on poly(L-lactic acid) (PLLA) films would influence the differentiation of human monocytes into macrophages. We tested PLLA films without modificat...
Multilayered films produced by layer-by-layer assembly of chitosan and alginate...
Hatami, Javad; Silva, Sandra G.; Oliveira, Mariana Braga; Costa, Rui R.; Reis, R. L.; Mano, J. F.The construction of multilayered films with tunable properties could offer new routes to produce biomaterials as a platform for 3D cell cultivation. In this study, multilayered films produced with five bilayers of chitosan and alginate (CHT/ALG) were built using water-soluble modified mesyl and tosylâ CHT via layer-by-layer (LbL) self-assembly. NMR results demonstrated the presences of mesyl (2.83 ppm) and tosy...
Screening of nanocomposite scaffolds arrays using superhydrophobic-wettable mic...
Leite, Álvaro J.; Oliveira, Mariana Braga; Caridade, Sofia Glória Ferreira; Mano, J. F.Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benc...
Fabrication and characterization of Eri silk fibers-based sponges for biomedica...
Silva, Simone Santos; Oliveira, Nuno M.; Oliveira, Mariana Braga; Costa, Diana Soares da; Naskar, Deboki; Mano, J. F.; Kundu, S. C.; Reis, R. L.Cocoon-derived semi-domesticated Eri silk fibers still lack exploitation for tissue engineering applications due to their poor solubility using conventional methods. The present work explores the ability to process cocoon fibers of non-mulberry Eri silk (Samia/Philosamia ricini) into sponges through a green approach using ionic liquid (IL) â 1-buthyl-imidazolium acetate as a solvent. The formation of β-sheet s...
BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
Ribeiro, Artur J.; Volkov, Vadim; Oliveira, Mariana Braga; Padrão, Jorge; Mano, J. F.; Gomes, Andreia C; Cavaco-Paulo, ArturAn effective tissue engineering approach requires adjustment according to the target tissue to be engineered. The possibility of obtaining a protein-based formulation for the development of multivalent tunable scaffolds that can be adapted for several types of cells and tissues is explored in this work. The incremental substitution of bovine serum albumin (BSA) by human serum albumin (HSA), changing the scaffol...
Biomimetic superhydrophobic surfaces patterned with wettable spots as implantab...
Oliveira, Mariana Braga; Ribeiro, Maximiano P.; Miguel, Sónia P.; Neto, Ana I.; Coutinho, Paula; Correia, Ilídio J.; Mano, J. F.Flat patterned superhydrophobic films are herein reported as implantable platforms containing arrays of miniaturized porous scaffolds for the high-throughput in vivo study of biomaterials. Highthroughput platforms are powerful platforms to detect hit conditions showing promising properties. However, high-content analysis of biomaterials has been mostly limited to in vitro testing where crucial information is lo...
Combinatorial effect of silicon and calcium release from starch-based scaffolds...
Rodrigues, Ana I.; Oliveira, Mariana Braga; Mano, J. F.; Gomes, Manuela E.; Reis, R. L.; Leonor, I. B.Instructive materials able to drive cells, in particular the differentiation of stem cells toward osteoblastic lineages, have been investigated as a promising strategy for bone tissue engineering. Inorganic ions, such as phosphorus, calcium, silicon, and strontium, have been used in bone regeneration strategies as instructive ions for material-based approaches. The use of effective inorganic ions is being inves...
Influence of clay organic modifier on the thermal-stability of PLA based nanoco...
Araújo, Andreia Isabel Silva; Botelho, Gabriela; Oliveira, Mariana Braga; Machado, A. V.Poly(lactic acid) (PLA) is a biodegradable aliphatic thermoplastic polyester well known for being a promising alternative to petroleum-based materials since it can be produced from renewable resources. Although this polymer has good properties when compared to other biodegradable polymers, it presents some limitations like poor thermal, mechanical resistance and gas barrier. The incorporation of clay minerals h...