12 documents found, page 1 of 2
Maternal heart exhibits metabolic and redox adaptations post-uncomplicated preg...
Tocantins, Carolina; Martins, João D.; Rodrigues, Óscar M; Grilo, Luís F.; Diniz, Mariana S.; Stevanovic-Silva, Jelena; Beleza, Jorge; Coxito, PedroPregnancy may be a challenging period for the maternal systems and has been regarded as a stress test, as imperceptible/mild dysfunctions eventually present may be exacerbated during this period. The cardiovascular system is no exception, and several morphological and functional adaptations accompanying pregnancy have been described. However, long-term pregnancy-induced cardiac molecular alterations remain high...
Maternal hepatic adaptations during obese pregnancy encompass lobe-specific mit...
Grilo, Luís F; Martins, João D; Diniz, Mariana S.; Tocantins, Carolina; Cavallaro, Chiara; Baldeiras, Inês; Cunha-Oliveira, Teresa; Ford, StephenMaternal obesity (MO) is rising worldwide, affecting half of all gestations, constituting a possible risk-factor for some pregnancy-associated liver diseases (PALD) and hepatic diseases. PALD occur in approximately 3% of pregnancies and are characterized by maternal hepatic oxidative stress (OS) and mitochondrial dysfunction. Maternal hepatic disease increases maternal and fetal morbidity and mortality. Underst...
Metabolic mitochondrial alterations prevail in the female rat heart 8 weeks aft...
Tocantins, Carolina; Martins, João D; Rodrigues, Óscar M; Grilo, Luís F; Diniz, Mariana S.; Stevanovic-Silva, Jelena; Beleza, Jorge; Coxito, PedroThe consumption of high-caloric diets strongly contributes to the development of non-communicable diseases (NCDs), including cardiovascular disease, the leading cause of mortality worldwide. Exercise (along with diet intervention) is one of the primary non-pharmacological approaches to promote a healthier lifestyle and counteract the rampant prevalence of NCDs. The present study evaluated the effects of exercis...
Fetoplacental endothelial dysfunction in gestational diabetes mellitus and mate...
Diniz, Mariana S.; Hiden, Ursula; Falcão-Pires, Inês; Oliveira, Paulo J; Sobrevia, Luis; Pereira, Susana PGestational diabetes mellitus (GDM) and maternal obesity (MO) increase the risk of adverse fetal outcomes, and the incidence of cardiovascular disease later in life. Extensive research has been conducted to elucidate the underlying mechanisms by which GDM and MO program the offspring to disease. This review focuses on the role of fetoplacental endothelial dysfunction in programming the offspring for cardiovascu...
The Alterations of Mitochondrial Function during NAFLD Progression-An Independe...
Simões, Inês C M; Amorim, Ricardo; Teixeira, José; Karkucinska-Wieckowska, Agnieszka; Carvalho, Adriana; Pereira, Susana P; Simões, Rui F.The progression of non-alcoholic fatty liver (NAFL) into non-alcoholic steatohepatitis implicates multiple mechanisms, chief of which is mitochondrial dysfunction. However, the sequence of events underlying mitochondrial failure are still poorly clarified. In this work, male C57BL/6J mice were fed with a high-fat plus high-sucrose diet for 16, 20, 22, and 24 weeks to induce NAFL. Up to the 20th week, an early m...
Maternal high-fat high-sucrose diet and gestational exercise modulate hepatic f...
Stevanović-Silva, Jelena; Beleza, Jorge; Coxito, Pedro; Pereira, Susana; Rocha, Hugo; Gaspar, Tiago Bordeira; Gärtner, Fátima; Correia, RossanaMaternal high-caloric nutrition and related gestational diabetes mellitus (GDM) are associated with a high-risk for developing metabolic complications later in life and in their offspring. In contrast, exercise is recognized as a non-pharmacological strategy against metabolic dysfunctions associated to lifestyle disorders. Therefore, we investigated whether gestational exercise delays the development of metabol...
Metabolic Disease Programming: From Mitochondria to Epigenetics, Glucocorticoid...
Grilo, Luís F; Tocantins, Carolina; Diniz, Mariana S.; Gomes, Rodrigo Mello; Oliveira, Paulo J; Matafome, Paulo Nuno Centeio; Pereira, Susana PEmbryonic and foetal development are critical periods of development in which several environmental cues determine health and disease in adulthood. Maternal conditions and an unfavourable intrauterine environment impact foetal development and may programme the offspring for increased predisposition to metabolic diseases and other chronic pathologic conditions throughout adult life. Previously, non-communicable ...
P-cadherin induces anoikis-resistance of matrix-detached breast cancer cells by...
Sousa, Bárbara; Pereira, Joana; Marques, Ricardo; Grilo, Luís F; Pereira, Susana P; Oliveira, Vilma Marisa Arrojado Soares Sardão; Schmitt, FernandoSuccessful metastatic spreading relies on cancer cells with stem-like properties, glycolytic metabolism and increased antioxidant protection, allowing them to escape anoikis and to survive in circulation. The expression of P-cadherin, a poor prognostic factor in breast cancer, is associated with hypoxic, glycolytic and acidosis biomarkers. In agreement, P-cadherin-enriched breast cancer cell populations present...
Development of a 96-well based assay for kinetic determination of catalase enzy...
Grilo, Luís F; Martins, João D; Cavallaro, Chiara H; Nathanielsz, Peter W; Oliveira, Paulo J; Pereira, Susana POxidative stress biomarkers are powerful endpoints in toxicological research. Cellular reductive/oxidative balance affects numerous signaling pathways involving H2O2. Detoxification and control of H2O2 levels results mainly from catalase activity. The aim of this work was to develop a precise, simple, cost-effective microassay to measure catalase activity in small tissue samples and cell extracts. We developed ...
Early Cardiac Mitochondrial Molecular and Functional Responses to Acute Anthrac...
Pereira, Gonçalo Castro; Pereira, Susana P.; Pereira, Francisco B.; Lourenço, Nuno António Marques; Lumini, José A; Pereira, Cláudia V.; Bjork, James A.Doxorubicin (DOX) is an anticancer drug widely used to treat human and nonhuman tumors but the late and persistent cardio-toxicity reduces the therapeutic utility of the drug. The full mechanism(s) of DOX-induced acute, subchronic and delayed toxicity, which has a preponderant mitochondrial component, remains unclear; therefore, it is clinically relevant to identify early markers to identify patients who are pr...