28 documents found, page 1 of 3
Energetics of Male Reproduction
Bastos, Ana; Alves, Marco G.; Silva, Branca M.; Oliveira, Pedro F.; Rato, LuísSpermatogenesis is a finely tuned process marked by specific metabolic adjustments at each stage of germ cell differentiation and sperm production. The Sertoli cell, often referred to as the "nurse cell," assumes a pivotal role in furnishing germ cells with the requisite metabolic substrates for their sustenance and differentiation. This dynamic establishes a continual metabolic partnership between Sertoli cell...
Differential Proteomic Analysis of Human Sperm: A Systematic Review to Identify...
Corda, Pedro O.; Moreira, Jéssica; Howl, John; Oliveira, Pedro F.; Fardilha, Margarida; Silva, Joana VieiraThe advent of proteomics provides new opportunities to investigate the molecular mechanisms underlying male infertility. The selection of relevant targets based on a single analysis is not always feasible, due to the growing number of proteomic studies with conflicting results. Thus, this study aimed to systematically review investigations comparing the sperm proteome of normozoospermic and infertile men to def...
Exenatide and Dapagliflozin Combination Enhances Sertoli Cell Secretion of Key ...
Ribeiro, João C.; Martins, Ana D.; Jarak, Ivana; Carvalho, Rui A.; Alves, Marco G.; Oliveira, Pedro F.The incidence of metabolic diseases such as type 2 diabetes mellitus (DM) and obesity has been increasing dramatically. Both diseases are closely linked and new approaches for type 2 DM treatment aim to enable weight loss. A combined therapy of dapagliflozin and exenatide has been used against type 2 DM, influencing allbody glucose dynamics. Spermatogenesis is highly dependent on the metabolic cooperation estab...
Hyperoside Supplementation in Preservation Media Surpasses Vitamin C Protection...
Moreira, Mafalda V; Pereira, Sara C; Guerra-Carvalho, Bárbara; Carrageta, David F; Pinto, Soraia; Barros, Alberto; Silva, Branca M.; Oliveira, Pedro F.Oxidative Stress (OS) is reported as one of the main causes of male infertility. Infertile couples often resort to assisted reproductive technology (ART) to achieve parenthood. However, preparation for ART protocols increases the exposer of gametes to OS. Thus, it is crucial to find suitable preservation media that can counteract the OS-induced damages in spermatozoa. In this work, we tested and compared the ef...
Effects of Age and Lifelong Moderate-Intensity Exercise Training on Rats' Testi...
Silva, Joana V.; Santiago, Joana; Matos, Bárbara; Henriques, Magda C.; Patrício, Daniela; Martins, Ana D.; Duarte, José A.; Ferreira, RitaAging is associated with testicular morphological and functional alterations, but the underlying molecular mechanisms and the impact of physical exercise are poorly understood. In this study, we examined the effects of age and lifelong moderate-intensity exercise on rat testis. Mature adults (35 weeks) and middle-aged (61 weeks) Wistar Unilever male rats were maintained as sedentary or subjected to a lifelong m...
PP1, PP2A and PP2B Interplay in the Regulation of Sperm Motility: Lessons from ...
Ferreira, Ana F.; Santiago, Joana; Silva, Joana V.; Oliveira, Pedro F.; Fardilha, MargaridaMale fertility relies on the ability of spermatozoa to fertilize the egg in the female reproductive tract (FRT). Spermatozoa acquire activated motility during epididymal maturation; however, to be capable of fertilization, they must achieve hyperactivated motility in the FRT. Extensive research found that three protein phosphatases (PPs) are crucial to sperm motility regulation, the sperm-specific protein phosp...
Intermittent Hypoxic Conditioning Rescues Cognition and Mitochondrial Bioenerge...
Correia, Sónia C.; Machado, Nuno J.; Alves, Marco G; Oliveira, Pedro F.; Moreira, Paula I.The lack of effective disease-modifying therapeutics to tackle Alzheimer's disease (AD) is unsettling considering the actual prevalence of this devastating neurodegenerative disorder worldwide. Intermittent hypoxic conditioning (IHC) is a powerful non-pharmacological procedure known to enhance brain resilience. In this context, the aim of the present study was to investigate the potential long-term protective i...
Inheritable testicular metabolic memory of high-fat diet causes transgeneration...
Crisóstomo, Luís; Jarak, Ivana; Rato, Luís P; Raposo, João F; Batterham, Rachel L; Oliveira, Pedro F.; Alves, Marco GThe consumption of energy-dense diets has contributed to an increase in the prevalence of obesity and its comorbidities worldwide. The adoption of unhealthy feeding habits often occurs at early age, prompting the early onset of metabolic disease with unknown consequences for reproductive function later in life. Recently, evidence has emerged regarding the intergenerational and transgenerational effects of high-...
Plasmatic Oxidative and Metabonomic Profile of Patients with Different Degrees ...
Silva-Vaz, Pedro; Jarak, Ivana; Rato, Luis; Oliveira, Pedro F.; Morgado-Nunes, Sara; Paulino, Aida; Castelo-Branco, Miguel; Botelho, FilomenaAcute pancreatitis (AP) is an inflammatory process of the pancreas with variable involvement of the pancreatic and peripancreatic tissues and remote organ systems. The main goal of this study was to evaluate the inflammatory biomarkers, oxidative stress (OS), and plasma metabolome of patients with different degrees of biliary AP severity to improve its prognosis. Twenty-nine patients with biliary AP and 11 heal...
Lifestyle, metabolic disorders and male hypogonadism - a one-way ticket?
Crisóstomo, Luís; Pereira, Sara C.; Monteiro, Mariana P.; Raposo, João F.; Oliveira, Pedro F.; Alves, Marco G.Hypogonadism is more frequent among men with common metabolic diseases, notably obesity and type 2 diabetes. Indeed, endocrine disruption caused by metabolic diseases can trigger the onset of hypogonadism, although the underlying molecular mechanisms are not entirely understood. Metabolic diseases are closely related to unhealthy lifestyle choices, such as dietary habits and sedentarism. Therefore, hypogonadism...