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An overview of the recent advances in antimicrobial resistance
Oliveira, Manuela; Antunes, Wilson; Mota, Salete; Carvalho, Áurea Madureira; Oliveira, Ricardo Jorge Dinis; Silva,Diana Dias daAntimicrobial resistance (AMR), frequently considered a major global public health threat, requires a comprehensive understanding of its emergence, mechanisms, advances, and implications. AMR’s epidemiological landscape is characterized by its widespread prevalence and constantly evolving patterns, with multidrug-resistant organisms (MDROs) creating new challenges every day. The most common mechanisms underlyin...
Optimization of the derivatization procedure for the separation of the stereois...
Almeida, Maria Mexia de; Silva, Diana Dias da; Oliveira, Ricardo Jorge Dinis; Ribeiro, Cláudia; Dias da Silva, Diana Cristina1,3-Dimethylamylamine (1,3-DMAA),also known as methylhexanamine, is a central nervous system stimulant with structural similarities with amphetamines and therefore presenting over-lapping biological and detrimental effects [1]. Despite being banned, the presence of 1,3-DMAA in dop-ing controls and dietary supplements continues to be of significant concern. This molecule has two stere-ogenic centres and thus fou...
Sequencing technologies in forensic microbiology: current trends and advancements
Oliveira, Manuela; Dias da Silva, Diana Cristina; Marszałek, Kamila; Kowalski, Michał; Frolova, Alina; Łabaj, Paweł P.; Branicki, WojciechForensic microbiology is a subject of interest and research development across the forensic community. Studies of pathogen outbreaks, biocrime or bioterrorism attacks, and analyses of crime scenes fall within this field. Significant progress has been made in evolving DNA sequencing technologies in recent decades. These newly emerged transformative tools have become available to both biomedicine and forensics. B...
Mapping of key bacterial species for Postmorteminterval calculation
Ferreira, Ana Cláudia; Barbosa, Daniel J.; Campos, Carla; Oliveira, Ricardo Jorge Dinis; Freitas, Ana R.Estimation of the PostmortemInterval (PMI), the time elapsed since death, is one of the most challenging issues in forensic sciences. Most studies focus on extensive bacterial sequencing, but culture-based experiments for higher taxonomic resolution remain scarce. We aimed to analyze total bacterial counts and map Enterococcus faecalis, Staphylococcus aureusand Escherichia coliin different organs and timepoints...
A breakthrough on Amanita phalloides poisoning: an effective antidotal effect b...
Garcia, Juliana; Costa, Vera Marisa; Carvalho, Alexandra T. P.; Silvestre, Ricardo Jorge Leal; Duarte, José Alberto; Dourado, Daniel F. A. R.Amanita phalloides is responsible for more than 90 % of mushroom-related fatalities, and no effective antidote is available. a-Amanitin, the main toxin of A. phalloides, inhibits RNA polymerase II (RNAP II), causing hepatic and kidney failure. In silico studies included docking and molecular dynamics simulation coupled to molecular mechanics with generalized Born and surface area method energy decomposition on ...
Toxicity of Paraquat in Isolated Rat Lung. Search for Efficient Antidotes
Oliveira, Ricardo Jorge DinisThe immune evasion mechanisms of pathogenic trypanosomatids involve a multitude of phenomena such as the polyclonal activation of lymphocytes, cytokine modulation and the enhanced detoxification of oxygen reactive species. A trypanothione-cascade seems to be involved in the detoxification process. We have recently described and characterized a tryparedoxin (LiTXN1) involved in the Leishmania infantum cytoplasma...