4 documents found, page 1 of 1
Use of Tunable Copolymers in Aqueous Biphasic Systems for Extractive Bioconvers...
Mendonça, Carlos M. N.; Veríssimo, Nathalia V.; Pereira, Wellison A.; Cunha, Paula M.; Vitolo, Michele; Converti, Attilio; Kurnia, Kiki AdiAqueous biphasic systems (ABSs) based on sodium polyacrylate (NaPA), ethylene oxide/propylene oxide (EO/PO) polymers, and (EO)x-(PO)y-(EO)x triblock copolymers were prepared and applied aiming at continuous fructooligosaccharide (FOS) production and separation. EO/PO hydrophilicity/hydrophobicity balance had a significant effect on ABS formation. To develop an integrated process including the continuous enzymat...
Characterization of levan produced by a Paenibacillus sp. isolated from Brazili...
Mendonça, Carlos M. N.; Oliveira, Rodrigo C.; Freire, Rominne K. B.; Piazentin, Anna C. M.; Pereira, Wellison A.; Gudiña, Eduardo JoséA levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear (26) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structure an...
Characterization of levan produced by a Paenibacillus sp. isolated from Brazili...
Mendonça, Carlos M. N.; Oliveira, Rodrigo C.; Freire, Rominne K. B.; Piazentin, Anna C. M.; Pereira, Wellison A.; Gudiña, Eduardo J.A levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear β(2 → 6) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structur...
An integrated process combining the reaction and purification of PEGylated prot...
Santos, João H. P. M.; Mendonça, Carlos M. N.; Silva, Amanda R. P.; Oliveira, Ricardo P. S.; Pessoa, Adalberto; Coutinho, João A. P.A downstream process combining PEGylation reaction and the use of enzyme conjugates acting as phase-forming components of aqueous biphasic systems (ABS) is proposed here. In this approach, citrate buffer (pH = 7.0) was used simultaneously to stop the reaction (avoiding the use of hydroxylamine) and as a phase forming agent inducing the phase separation of the PEGylated proteins. The partition of the bioconjugat...