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Fatores de risco genético para adenomas hipofisários: Uma análise nacional, mul...
Gaspar, Leonor Isabel MesquitaOs adenomas hipofisários representam, aproximadamente, 10-15% do total dos tumores intracranianos. A prevalência destes tumores foi estimada em 1:1000 na população geral, sendo mais frequentemente diagnosticados entre os 40-60 anos de idade. Estes tumores são monoclonais, tipicamente benignos e de crescimento lento, no entanto podem estar associados a um aumento da morbilidade e mortalidade através da sobreprod...
Refinement of two-dimensional electrophoresis for vitreous proteome profiling u...
Santos, Fátima Raquel Milhano dos; Albuquerque, Tânia Gonçalves; Gaspar, Leonor Isabel Mesquita; Dias, Joao ML; Sousa, João Paulo Castro DeDespite technological advances, two-dimensional electrophoresis (2DE) of biological fluids, such as vitreous, remains a major challenge. In this study, artificial neural network was applied to optimize the recovery of vitreous proteins and its detection by 2DE analysis through the combination of several solubilizing agents (CHAPS, Genapol, DTT, IPG buffer), temperature, and total voltage. The highest protein re...
iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal De...
Santos, F.M.; Gaspar, Leonor Isabel Mesquita; Ciordia, Sergio; Rocha, Ana; Sousa, João Paulo Castro De; Paradela, Alberto; Passarinha, LA; Tomaz, C. T.Rhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional information about the molecular mechanisms underlying its pathogenesis. In the present study, iTRAQ labeling was c...
Proteome analysis of vitreous humor in retinal detachment using two different f...
Gaspar, Leonor Isabel Mesquita; Santos, F.M.; Albuquerque, Tânia; Sousa, João P. Castro de; Passarinha, L A; Tomaz, Cândida T.The deeper understanding of retinal detachment (RD) pathogenesis may improve the visual outcome after surgery. Given the main role of the vitreous in retinal eye diseases, two strategies were explored to identify its proteome in RD. Fractionation techniques such as anion exchange chromatography (IEX) and SDS-PAGE combined with MALDI-TOF/TOF analysis allowed to identify 127 proteins in vitreous of RD patients. F...
Proteomic analysis of the human vitreous humor in Retinal Detachment
Gaspar, Leonor Isabel MesquitaOver the past few years, there has been an intensification in onset of ocular diseases among worldwide population. The incidence of eye pathologies upsurges with advancing age, leading to a decline of normal eye function and even to blindness. Ocular diseases are frequently triggered by chronic disorders, such as diabetes or hypertension, or by alterations in retinal positioning, which occurs in retinal detachm...