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Biochemistry and Renal Function: Use of Inquiry-Based Teaching Sequences for Re...
Magalhães,Prislaine Pupolin; Oliveira,Rodrigo Cardoso; Ponce,Daniela; Zuliani,Silvia Regina Quijadas AroABSTRACT Current teaching proposals involve teaching strategies that seek to assist and encourage the construction of knowledge by the students, turning them into active participants during the learning process and these have been the subject of several researches. This article reports a didactic sequence carried out with students of applied training for the first year Medical Sciences USP – Bauru, and proposes...
Cell density and solvent are critical parameters affecting formazan evaluation ...
Gasque,Kellen Cristina da Silva; Al-Ahj,Luana Polioni; Oliveira,Rodrigo Cardoso; Magalhães,Ana CarolinaThe aim of this study was to establish the more accurate protocol for fibroblast cell viability using MTT assay. NIH/3T3 fibroblasts were seeded at the following cell densities: 3.125x10³; 1.156x10(4); 3.125x10(4); 1.156x10(5) and 3.125x10(5) cells/cm². Following 24h of seeding, MTT was added to the wells. After 4h of the MTT addition, different solvents were added to solubilize the formazan crystals: 1) HCl/SD...
Exploring anorganic bovine bone granules as osteoblast carriers for bone bioeng...
Zambuzzi,Willian F.; Fernandes,Gustavo V. O.; Iano,Flávia G.; Fernandes,Mileni da S.; Granjeiro,José Mauro; Oliveira,Rodrigo CardosoIt is known that current trends on bone bioengineering seek ideal scaffolds and explore innovative methods to restore tissue function. In this way, the objective of this study was to evaluate the behavior of anorganic bovine bone as osteoblast carrier in critical-size calvarial defects. MC3T3-E1 osteoblast cells (1x10(5) cells/well) were cultured on granules of anorganic bovine bone in 24-well plates and after ...
Microscopic analysis of dog dental pulp after pulpotomy and pulp protection wit...
Menezes,Renato; Bramante,Clóvis Monteiro; Garcia,Roberto Brandão; Letra,Ariadne; Carvalho,Vanessa Graciela Gomes; Carneiro,Everdan; Brunini,SérgioConsidering previous studies on the similarity between the chemical composition of the mineral trioxide aggregate and the Portland cement, the purpose of this study was to investigate the pulp response of dog's teeth after pulpotomy and direct pulp protection with MTA Angelus and white Portland cement. Thirty eight pulp remnants were protected with these materials. One hundred and twenty days after treatment, t...