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Morphological, Molecular and Genomic Identification and Characterisation of Mon...
Baltazar, Elsa; Rodrigues, Sara; Ares, Aitana; Camelo, Alexandra; Brandão, Inês; Espirito Santo, Christophe; Trovão, João; Garcia, Eva; Costa, JoanaIn Portugal, the Cova da Beira region is well-known for the production of Prunus spp. and is considered the main peach production area in the country. In the spring of 2021 and 2022, field surveys in peach and nectarine orchards showed symptoms of decline such as cankers, gummosis, dry branches, abortion of flowers, mummified fruits and the partial or total death of some plants. Brown rot is caused by three spe...
Novel, Edible Melanin-Protein-Based Bioactive Films for Cheeses: Antimicrobial,...
Ferraz, Ana Rita; Goulão, Manuela; Espirito Santo, Christophe; Anjos, Ofélia; Serralheiro, Maria Luísa; Pintado, Cristina M. B. S.The cheese rind is the natural food packaging of cheese and is subject to a wide range of external factors that compromise the appearance of the cheese, including color defects caused by spoilage microorganisms. First, eight films based on whey protein isolate (WPI) coatings were studied, of which IS3CA (WPI 5% + sorbitol 3% + citric acid 3%) was selected for presenting better properties. From the IS3CA film, n...
An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Lo...
Cordeiro, Daniela; Camelo, Alexandra; Pedrosa, Ana Carolina; Brandão, Inês; Canhoto, Jorge; Espirito Santo, Christophe; Correia, SandraLong-read sequencing methods allow a comprehensive analysis of transcriptomes in identifying full-length transcripts. This revolutionary method represents a considerable breakthrough for non-model species since it allows enhanced gene annotation and gene expression studies when compared to former sequencing methods. However, woody plant tissues are challenging to the successful preparation of cDNA libraries, th...
Evaluation of comparative scenarios from different sites of chestnut production...
Pakpahan, Okta Pringga; Moreira, Liliana; Camelo, Alexandra; Karya, Detri; Martins, Ana Catarina; Gaspar, Pedro Dinis; Espirito Santo, ChristopheThe evaluation of the environmental impacts of chestnut production in the Beira Interior region (Portugal) is accessed. The comparative life cycle assessment (LCA) was performed with the use of openLCA software with 16 Environmental Footprint (EF) impact categories retrieved from the AGRIBALYSE database. The system boundary was from “cradle-to-farm gate” and the functional unit was 1 ton of chestnut delivered t...
Effect of Controlled Atmospheres and Environmental Conditions on the Physicoche...
Pinto de Andrade, Luís; Veloso, Abel; Espirito Santo, Christophe; Dinis Gaspar, Pedro; Silva, Pedro Dinho; Resende, Mafalda; Beato, HelenaSweet cherry is a highly appreciated seasonal fruit with a high content of bioactive compounds; however, this highly perishable fruit has a relatively short shelf-life period. Here, we evaluated the evolution of the physicochemical and sensory qualities of sweet cherries (Prunus avium (L.) cv. Satin) under different storage conditions, namely at a Farmers’ Organization (FO) and in a Research Centre (RC) under n...
Effect of Controlled Atmospheres and Environmental Conditions on the Physicoche...
Andrade, Luís Pinto de; Veloso, Abel; Espirito Santo, Christophe; Gaspar, Pedro Dinis; Silva, Pedro Dinho da; Resende, Mafalda; Beato, HelenaSweet cherry is a highly appreciated seasonal fruit with a high content of bioactive compounds; however, this highly perishable fruit has a relatively short shelf-life period. Here, we evaluated the evolution of the physicochemical and sensory qualities of sweet cherries (Prunus avium (L.) cv. Satin) under different storage conditions, namely at a Farmers’ Organization (FO) and in a Research Centre (RC) under n...
Gut Microbiota, in the Halfway between Nutrition and Lung Function
Espirito Santo, Christophe; Caseiro, Catarina; Martins, Maria João; Monteiro, Rosário; Brandão, InêsThe gut microbiota is often mentioned as a "forgotten organ" or "metabolic organ", given its profound impact on host physiology, metabolism, immune function and nutrition. A healthy diet is undoubtedly a major contributor for promoting a "good" microbial community that turns out to be crucial for a fine-tuned symbiotic relationship with the host. Both microbial-derived components and produced metabolites elicit...
Influence of storage conditions on fruit quality of ‘Royal Time’ and ‘Royal Sum...
Veloso, Abel; Ferreira, Dora; Gaspar, Pedro Dinis; Andrade, Luís Pinto de; Espirito Santo, Christophe; Silva, Pedro D.; Simões, Maria PaulaPeach is a very perishable climacteric fruit whose firmness may decay rapidly depending on the temperature. Refrigeration is usually used to delay ripening and maintain fruit quality. However, often temperature storage conditions are not optimized. This work aims to characterize the storage conditions (temperature and humidity) of three peach producers of Beira Interior region, Portugal. Additionally, correlate...
Antimicrobial metallic copper surfaces kill Staphylococcus haemolyticus via mem...
Espirito Santo, Christophe; Quaranta, Davide; Grass, GregorRecently, copper (Cu) in its metallic form has regained interest for its antimicrobial properties. Use of metallic Cu surfaces in worldwide hospital trials resulted in remarkable reductions in surface contaminations. Yet, our understanding of why microbes are killed upon contact to the metal is still limited and different modes of action have been proposed. This knowledge, however, is crucial for sustained use ...