6 documents found, page 1 of 1
Brassica oleracea Var italica by-Products Prevent Lipid Accumulation and Cell D...
Castelão-Baptista, José P.; Valente, Sara A.; Canário, Sara; Oppolzer, David; Barros, Ana; Venâncio, Carlos; Martins, Tânia; Antunes, LuísObesity, a rising concern in the Eastern world, encompasses several co-morbidities, namely non-alcoholic fatty liver disease (NAFLD). Potential natural-based interventions to decrease the burden of obesity complications are being investigated. Many of the edible parts of plants are not sold for consumption and end up as massive waste, losing nutritional potential. In fact, a sizeable amount of waste is generate...
Phenolic rich extracts from cowpea sprouts decrease cell proliferation and enha...
Guedes, Catarina I. Teixeira; Oppolzer, David; Barros, Ana I.; Pereira-Wilson, CristinaIt is known that diets high in legumes can help in the prevention and/or reduction of colorectal cancer. These effects may result, in part, from their high phenolic content. The aims of this study were to characterize the effect of sprouting on phenolic content and antioxidant activity, and test the anti-colorectal cancer effects including possible potentiation of 5-fluorouracil efficacy. Sprouting increased 1....
Impact of cooking method on phenolic composition and antioxidant potential of f...
Teixeira-Guedes, Catarina I.; Oppolzer, David; Barros, Ana I.; Pereira-Wilson, CristinaThe present study aimed to investigate the effects of different cooking conditions - atmospheric (100°C) and pressure cooking (115°C) - on the phenolic composition and antioxidant activity of methanolic extracts of four Phaseolus vulgaris varieties and soy (Glycine max). Contrary to soy, in P. vulgaris varieties both cooking methods increased drastically the total phenolic, flavonoid, and ortho-diphenol content...
Evaluation of Mut(S) and Mut⁺ Pichia pastoris strains for membrane-bound catech...
Pedro, Augusto; Oppolzer, David; Bonifácio, M J; Maia, C J; Queiroz, João; Passarinha, L ACatechol-O-methyltransferase (COMT, EC 2.1.1.6) is an enzyme that catalyzes the methylation of catechol substrates, and while structural and functional studies of its membrane-bound isoform (MBCOMT) are still hampered by low recombinant production, Pichia pastoris has been described as an attractive host for the production of correctly folded and inserted membrane proteins. Hence, in this work, MBCOMT biosynthe...
Combinatorial delivery of Crizotinib–Palbociclib–Sildenafil using TPGS-PLA mice...
Diogo, Duarte Miguel de Melo; Gaspar, Vítor Manuel Abreu; Costa, Elisabete C.; Moreira, André; Oppolzer, David; Gallardo, EugeniaThe co-delivery of multiple chemotherapeutics by micellar delivery systems is a valuable approach to improve cancer treatment since various disease hallmarks can be targeted simultaneously. However, the delivery of multiple drugs requires a nanocarrier structure that can encapsulate various bioactive molecules. In this study, we evaluate the simultaneous encapsulation of a novel triple drug combination in D-α-t...
Co-delivery of Sildenafil (Viagra®) and Crizotinib for Synergistic and Improved...
Marques, João Filipe Gonçalves; Gaspar, Vítor Manuel Abreu; Oppolzer, David; Costa, Elisabete C.; Gallardo, Eugenia; Correia, Ilídio Joaquim SobreiraPurpose Cancer multi-drug resistance is a major issue associated with current anti-tumoral therapeutics. In this work, Crizotinib an anti-tumoral drug approved for the treatment of non-small lung cancer in humans, and Sildenafil (Viagra®), were loaded into micellar carriers to evaluate the establishment of a possible synergistic anti-tumoral effect in breast cancer cells. Methods Micellar carriers comprised by ...