3 documents found, page 1 of 1
An anaphase surveillance mechanism prevents micronuclei formation from frequent...
Orr, B; Sousa, F; Gomes, AM; Afonso, O; Ferreira, LT; Figueiredo, AC; Maiato, HMicronuclei are a hallmark of cancer and several other human disorders. Recently, micronuclei were implicated in chromothripsis, a series of massive genomic rearrangements that may drive tumor evolution and progression. Here, we show that Aurora B kinase mediates a surveillance mechanism that integrates error correction during anaphase with spatial control of nuclear envelope reassembly to prevent micronuclei f...
Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 cros...
Afonso, O; Castellani, CM; Cheeseman, LP; Ferreira, JG; Orr, B; Ferreira, LT; Chambers, JJ; Morais-de-Sá, E; Maresca, TJ; Maiato, HAccording to the prevailing ‘clock’ model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at the metaphase-anaphase transition, controlled by the spindle assembly checkpoint. However, mitotic exit was recently shown to be a function of chromosome separation during anaphase, assisted by a midzone Aurora B phosphorylation gradient-the ‘ruler’...
Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator
Lince-Faria, M; Maffini, S; Orr, B; Ding, Y; Florindo, C; Sunkel, CE; Tavares, Á; Johansen, J; Johansen, KM; Maiato, HA putative spindle matrix has been hypothesized to mediate chromosome motion, but its existence and functionality remain controversial. In this report, we show that Megator (Mtor), the Drosophila melanogaster counterpart of the human nuclear pore complex protein translocated promoter region (Tpr), and the spindle assembly checkpoint (SAC) protein Mad2 form a conserved complex that localizes to a nuclear derived...