Eight presumably related dark red-berried (dR), light red-berried (lR) and white-berried (W) grapevine cultivars grown under similar environmental conditions were characterised on the basis of morphologic (phenotype) and molecular (genotype) parameters. The ampelographic characterisation was based on the main descriptors that correspond to the 'Primary descriptor priority list' suggested by the International Or...
Grapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of ‘Vinhos Verdes’ and ‘Douro’ Controlled Designations ...
A germplasm set of twenty-five grapevine accessions, forming eleven groups of possible berry skin color mutants, were genotyped with twelve microsatellite loci, being eleven of them identified as true color mutants. The polyphenolic profiling of the confirmed mutant cultivars revealed a total of twenty-four polyphenols, comprising non-colored compounds (phenolic acids, flavan-3-ols, flavonols and a stilbene) an...
Grapevine chloroplast (cp) DNA diversity was analysed for the first time through amplification and digestion of fragments of the large single copy (LSC)region by polymerase chain reaction–restriction fragment length polymorphism methodology and also by amplification of three microsatellite loci, previously described as polymorphic in grapevine. Thirty-eight grapevine cultivars collected mainly in the North of P...
Within Western Europe, Portugal is the country with highest diversity of grapevine cultivars. As the Iberian Peninsula has been considered a domestication centre of grapevines, the interest on Portuguese grapevine cultivars diversity has increased in the last years. Using twelve SSR markers scattered across twelve grape chromosomes, thirty-three accessions collected on Vinhos Verdes and Lafões Controlled Design...
Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 true-to-type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinhão cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based pol...
Thirty-nine grapevine cultivars widely grown in Portugal, especially in Vinhos Verdes and Douro regions,and two well known international cultivars as standards, were genotyped at 12 microsatellite loci. The number of alleles per locus ranged from 6 to 12, and the number of allelic combinations per locus from 13 to 26. The total number of unique genotypes in the 12 analysed loci was 120, having most of the culti...