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Microbiological quality and safety of raw milk and soft cheese and detection of...
Ortolani, Maria Beatriz Tassinari; Yamazi, Anderson Keizo; Moraes, Paula Mendonca; Nero, Luis Augusto; Viçosa, Gabriela NogueiraThis study aimed to characterize the microbiological quality and safety of raw milk and soft cheese, verifying possible associations between microbial populations, and the detection of lactic acid bacteria (LAB) with antagonistic activity against foodborne pathogens. Raw milk (n = 36) and soft cheese (n = 18) samples were collected and submitted for the analysis of mesophilic aerobes, total coliforms, Escherich...
Foodborne pathogens and microbiological characteristics of raw milk soft cheese...
Moraes, Paula Mendonca; Vicosa, Gabriela Nogueira; Yamazi, Anderson Keizo; Ortolani, Maria Beatriz Tassinari; Augusto Nero, LuisThe consumption of raw milk soft cheeses (RMSC), which are typically manufactured in small dairy farms under unsatisfactory hygiene conditions, is common in Brazil. Due to these production characteristics, this type of cheese is a potential carrier of pathogenic microorganisms, such as Listeria monocytogenes, Salmonella, and enterotoxin-producing Staphylococcus spp. Considering these characteristics, in this wo...
Protocols for the isolation and detection of lactic acid bacteria with bacterio...
Moraes, Paula Mendonça; Perin, Luana Martins Perin; Ortolani, Maria Beatriz Tassinari; Yamazi, Anderson Keizo; Viçosa, Gabriela NogueiraThe objective of this study was to evaluate culture media and methodologies for isolation and detection of lactic acid bacteria (LAB) capable to produce bacteriocin-like substances. Samples of milk and cheese were pour plated on de Mann-Rogosa-Sharpe agar (MRS) and Kang-Fung-Sol agar (KFS) (both at 35 °C/48 h, under anaerobiosis), from which 389 and 256 LAB cultures were selected. The antagonistic activity of t...
Bactérias ácido láticas autóctones de leite cru e queijo minas frescal: isolame...
Ortolani, Maria Beatriz TassinariThe poor microbiological quality of Brazilian raw milk as a result of poor hygienic conditions usually observed during the production. As consequence, raw milk and dairy products present high levels of hygiene indicators microorganisms, suggesting the presence of pathogens. However, pathogens have low competitive ability, suffering direct interference of the autochthonous microbiota of these products. This inte...
Evaluation of the therapeutic supplementation with commercial powder probiótic ...
Camargo, Pedro Luiz de; Ortolani, Maria Beatriz Tassinari; Uenaka, Simone Akemi; Motta, Maitê Bette; Braga, Cecília dos ReisHemorrhagic gastroenteritis (GHE) is a common motivation to hospitalization and mortality between puppies. Considering the beneficial effects of probiotics in the treatment of some gastrointestinal disorders, we evaluated the usefulness of the probiotic product in 100 puppies hospitalized because GHE. Fifth dogs were treated by intravenous fluid, antiemetic, antibiotics and probiotic product (G1), and the other...