7 documents found, page 1 of 1
Equatorial Non-muscle Myosin II and Plastin Cooperate to Align and Compact F-ac...
Leite, J; Chan, FY; Osório, DS; Saramago, J; Sobral, AF; Silva, AM; Gassmann, R; Carvalho, AXCytokinesis is the last step of cell division that physically partitions the mother cell into two daughter cells. Cytokinesis requires the assembly and constriction of a contractile ring, a circumferential array of filamentous actin (F-actin), non-muscle myosin II motors (myosin), and actin-binding proteins that forms at the cell equator. Cytokinesis is accompanied by long-range cortical flows from regions of r...
Dullard-mediated smad1/5/8 inhibition controls mouse cardiac neural crest cells...
Darrigrand, JF; Valente, M; Comai, G; Martinez, P; Petit, M; Nishinakamura, R; Osório, DS; Renault, G; Marchiol, C; Ribes, V; Cadot, BThe establishment of separated pulmonary and systemic circulation in vertebrates, via the cardiac outflow tract (OFT) septation is a sensitive developmental process accounting for 10% of all congenital anomalies. Neural Crest Cells (NCC) colonising the heart condensate along the primitive endocardial tube and force its scission into two tubes. Here, we show that NCC aggregation progressively decreases along the...
PIG-1 MELK-dependent phosphorylation of nonmuscle myosin II promotes apoptosis ...
Wei, H; Lambie, EJ; Osório, DS; Carvalho, AX; Conradt, BThe mechanism(s) through which mammalian kinase MELK promotes tumorigenesis is not understood. We find that the C. elegans orthologue of MELK, PIG-1, promotes apoptosis by partitioning an anti-apoptotic factor. The C. elegans NSM neuroblast divides to produce a larger cell that differentiates into a neuron and a smaller cell that dies. We find that in this context, PIG-1 MELK is required for partitioning of CES...
Network contractility during cytokinesis-from molecular to global views
Leite, J; Osório, DS; Sobral, AF; Silva, AM; Carvalho, AXCytokinesis is the last stage of cell division, which partitions the mother cell into two daughter cells. It requires the assembly and constriction of a contractile ring that consists of a filamentous contractile network of actin and myosin. Network contractility depends on network architecture, level of connectivity and myosin motor activity, but how exactly is the contractile ring network organized or interco...
TPXL-1 activates Aurora A to clear contractile ring components from the polar c...
Mangal, S; Sacher, J; Kim, T; Osório, DS; Motegi, F; Carvalho, AX; Oegema, K; Zanin, EDuring cytokinesis, a signal from the central spindle that forms between the separating anaphase chromosomes promotes the accumulation of contractile ring components at the cell equator, while a signal from the centrosomal microtubule asters inhibits accumulation of contractile ring components at the cell poles. However, the molecular identity of the inhibitory signal has remained unknown. To identify molecular...
Emerging Mechanisms and Roles for Asymmetric Cytokinesis
Thieleke-Matos, C; Osório, DS; Carvalho, AX; Morais-de-Sá, ECytokinesis completes cell division by physically separating the contents of the mother cell between the two daughter cells. This event requires the highly coordinated reorganization of the cytoskeleton within a precise window of time to ensure faithful genomic segregation. In addition, recent progress in the field highlighted the importance of cytokinesis in providing particularly important cues in the context...
Robust gap repair in the contractile ring ensures timely completion of cytokine...
Silva, AM; Osório, DS; Pereira, AJ; Maiato, H; Pinto, IM; Rubinstein, B; Gassmann, R; Telley, IA; Carvalho, AXCytokinesis in animal cells requires the constriction of an actomyosin contractile ring, whose architecture and mechanism remain poorly understood. We use laser microsurgery to explore the biophysical properties of constricting rings in Caenorhabditis elegans embryos. Laser cutting causes rings to snap open. However, instead of disintegrating, ring topology recovers and constriction proceeds. In response to sev...