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Wolbachia screening in Aedes aegypti and Culex pipiens mosquitoes from Madeira ...

Fernandes, Rita; Melo, Tiago; Zé-Zé, Líbia; Freitas, Inês C.; Silva, Manuel; Dias, Eva; Santos, Nuno C.; Gouveia, Bruna; Seixas, Gonçalo; Osório, Hugo

Mosquito-borne diseases such as dengue and West Nile virus pose serious public health risks. On Madeira Island, the presence of the mosquito species Aedes aegypti (Linnaeus, 1762) and Culex pipiens (Linnaeus, 1758) raises concerns about local transmission. In this study, we tested 100 Ae. aegypti and 40 Cx. pipiens mosquitoes collected exclusively in the municipality of Funchal, Madeira Island, to assess the pr...


Red blood cell proteomic profiling in mild and severe obstructive sleep apnea p...

Valentim-Coelho, Cristina; Saraiva, Joana; Osório, Hugo; Antunes, Marilia; Vaz, Fátima; Neves, Sofia; Pinto, Paula; Bárbara, Cristina; Penque, Deborah

Obstructive Sleep Apnea (OSA) is characterized by recurrent-episodes of apneas/hypopneas during sleep, leading to recurrent intermittent-hypoxia and sleep fragmentation. Non-treated OSA can result in cardiometabolic diseases. In this study, we applied a shotgun-proteomics strategy to deeper investigate the red blood cell-(RBC) homeostasis regulation in the context of OSA-severity and their response to six month...


Red blood cell proteomic profiling in mild and severe obstructive sleep apnea p...

Valentim-Coelho, Cristina; Saraiva, Joana; Osório, Hugo; Antunes, Marília; Vaz, Fátima; Neves, Sofia; Pinto, Paula; Bárbara, Cristina; Penque, Deborah

Obstructive Sleep Apnea (OSA) is characterized by recurrent-episodes of apneas/hypopneas during sleep, leading to recurrent intermittent-hypoxia and sleep fragmentation. Non-treated OSA can result in cardiometabolic diseases. In this study, we applied a shotgun-proteomics strategy to deeper investigate the red blood cell-(RBC) homeostasis regulation in the context of OSA-severity and their response to six month...


Toscana Virus in Wild-Caught Sand Flies in Portugal, Findings from the National...

Amaro, Fátima; Amaro F.; Zé-Zé, Líbia; Osório, Hugo; Soares, Patricia; Silva, Manuel; Freitas, Inês Campos; Alves, Maria João

Phlebotomine sand flies play a crucial role in both human and veterinary medicine, acting as vectors for Leishmania parasites and most known phleboviruses. In Portugal, the REVIVE program, a comprehensive national surveillance network under the Ministry of Health, has included sand fly surveys since 2016. REVIVE aims to identify existing sand fly species in the country, determine which pathogens are circulating...


The spread of the invasive mosquito Aedes albopictus (Diptera: Culicidae) in Po...

Zé-Zé, Líbia; Campos Freitas, Inês; Silva, Manuel; Soares, Patricia; Alves, Maria João; Osório, Hugo

Background: Aedes albopictus, commonly known as the Asian tiger mosquito, has become one of the most invasive mosquito species. Over the last 5 decades, it has been introduced and established in various tropical and temperate regions worldwide. First reported in Europe in 1979 in Albania and later in Italy in 1990, the species is now established in 13 European Union (EU)/European Economic Area (EEA) countries a...


Mark–Release–Recapture Trial with Aedes albopictus (Diptera, Culicidae) Irradia...

Amaro, Fátima; Amaro F.; Soares, Patricia; Velo, Enkelejda; Oliveira Carvalho, Danilo; Gomez, Maylen; Balestrino, Fabrizio; Puggioli, Arianna

Aedes albopictus is considered one of the major invasive species in the world and can transmit viruses such as dengue, Zika, or chikungunya. The Sterile Insect Technique (SIT) can be used to suppress the native populations of Ae. albopictus. Mark–release–recapture (MRR) studies are crucial to support the development of the release strategy during the SIT application. Meanwhile, weather conditions can affect the...


Biofilm matrix regulation by Candida glabrata Zap1 under acidic conditions: tra...

Gonçalves, Bruna Fernandes; Pires, Diana Priscila Penso; Fernandes, L.; Pacheco, Miguel; Ferreira, Tiago; Osório, Hugo; Soares, Ana Raquel

The vaginal acidic environment potentiates the formation of Candida glabrata biofilms, leading to complicated and recurrent infections. Importantly, the production of matrix is known to contribute to the recalcitrant features of Candida biofilms. In this study, we reveal that Zap1 regulates the matrix of C. glabrata acidic biofilms and analyzed the modulation of their transcriptome (by microarrays) and matrix p...


Mosquito (Diptera: Culicidae) Fauna of a Zoological Park in an Urban Setting: A...

Madeira, Sara; Osório, Hugo; Boinas, Fernando; Bernardino, Rui

Mosquito-borne diseases (MBDs) are important emerging diseases that affect humans and animals. Zoological parks can work as early warning systems for the occurrence of MBDs. In this study, we characterized the mosquito fauna captured inside Lisbon Zoo from May 2018 to November 2019. An average of 2.4 mosquitos per trap/night were captured. Five mosquito species potentially causing MBDs, including Culex pipiens ...


Rede de Vigilância de Vetores - Flebótomos: a importância da vigilância de um v...

Amaro, Fátima; Osório, Hugo; Silva, Manuel; Freitas, Inês Campos; Soares, Patrícia; Vilares, Anabela; Martins, Susana; Reis, Tânia; Gargaté, Maria João

O programa REVIVE (Rede de Vigilância de Vetores) resulta de colaboração entre a Direção-Geral da Saúde, as Administrações Regionais de Saúde do Algarve, Alentejo, Centro, Lisboa e Vale do Tejo e Norte, a Direção Regional da Saúde da Madeira, a Direção Regional da Saúde dos Açores e o Instituto Nacional de Saúde Doutor Ricardo Jorge. No âmbito do REVIVE é realizada a vigilância entomológica, a nível nacional, d...


Field evaluation of an automated mosquito surveillance system which classifies ...

González-Pérez, María I.; Faulhaber, Bastian; Aranda, Carles; Williams, Mark; Villalonga, Pancraç; Silva, Manuel; Osório, Hugo; Encarnaçao, Joao

Background: Mosquito-borne diseases are a major concern for public and veterinary health authorities, highlighting the importance of effective vector surveillance and control programs. Traditional surveillance methods are labor-intensive and do not provide high temporal resolution, which may hinder a full assessment of the risk of mosquito-borne pathogen transmission. Emerging technologies for automated remote ...


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