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Toscana Virus in Wild-Caught Sand Flies in Portugal, Findings from the National...

Amaro, Fátima; Zé-Zé, Líbia; Osório, Hugo Costa; Soares, Patrícia; Silva, Manuel; Freitas, Inês Campos; Alves, Maria João

Phlebotomine sand flies play a crucial role in both human and veterinary medicine, acting as vectors for Leishmania parasites and most known phleboviruses. In Portugal, the REVIVE program, a comprehensive national surveillance network under the Ministry of Health, has included sand fly surveys since 2016. REVIVE aims to identify existing sand fly species in the country, determine which pathogens are circulating...


Residual Longevity of Recaptured Sterile Mosquitoes as a Tool to Understand Fie...

Balatsos, Georgios; Blanco-Sierra, Laura; Karras, Vasileios; Puggioli, Arianna; Osório, Hugo Costa; Bellini, Romeo; Papachristos, Dimitrios P.

Invasive mosquito species, such as Aedes albopictus, pose significant threats to both ecosystems and public health due to their role in transmitting diseases, such as dengue, Zika, and chikungunya. The Sterile Insect Technique (SIT) is a promising vector control strategy aimed at reducing mosquito populations by releasing sterile males to mate with wild females and reduce their reproduction rates. In this study...


Mosquito (Diptera: Culicidae) Fauna of a Zoological Park in an Urban Setting: A...

Madeira, Sara; Bernardino, Rui; Osório, Hugo Costa; Boinas, Fernando

Simple Summary: Mosquitoes are known for being a nuisance but also as important vectors of disease agents that affect not only humans but also animals. Zoological gardens are special places where humans and animals are found in close proximity, and where mosquitoes can also find the conditions required for their life cycle. This can be especially true for zoos located in urban areas. In this study, we character...


REVIVE 2023 e riscos para a saúde pública em Portugal: vigilância de carraças e...

Carvalho, Isabel Lopes de; Sousa, Rita de; Luz, Teresa; Parreira, Paulo; Gomes, Maria Salomé; Soares, Patrícia; Zé-Zé, Líbia; Osório, Hugo Costa

As carraças são um dos vetores mais importantes de agentes patogénicos para o Homem, com impacto crescente em saúde pública em todo o mundo. Os agentes infeciosos transmitidos por carraças incluem vírus, como o vírus da encefalite transmitida por carraças e da febre hemorrágica Crimeia-Congo, bactérias, tais como os agentes etiológicos da borreliose de Lyme, febre escaro nodular, outras rickettsioses e anaplasm...


Co-Circulation of Leishmania Parasites and Phleboviruses in a Population of San...

Amaro, Fátima; Vilares, Anabela; Martins, Susana; Reis, Tânia; Osório, Hugo Costa; Alves, Maria João; Gargaté, Maria João

In the Old World, phlebotomine sand flies from the genus Phlebotomus are implicated in the transmission of Leishmania spp. parasites (Kinetoplastida: Trypanosomatidae) and viruses belonging to the genus Phlebovirus (Bunyavirales: Phenuiviridae). Two of the five sand fly species known to occur in Portugal, Phlebotomus perniciosus and Ph. ariasi, the former being the most ubiquitous, are recognized vectors of Lei...


Vigilância automatizada e remota de mosquitos na Rede de Vigilância de Vetores ...

Osório, Hugo Costa; Silva, Manuel; Encarnação, João; Zé-Zé, Líbia; Amaro, Fátima; Alves, Maria João

No âmbito da Rede de Vigilância de Vetores (REVIVE ), é realizada a vigilância entomológica em mosquitos desde 2008 em Portugal. O projeto REVIVE é uma componente dos programas de vigilância epidemiológica indispensável à avaliação do risco de transmissão de agentes patogénicos transmitidos por vetores e é fundamental para a implementação de uma estratégia integrada de controlo de vetores e prevenção de doenças...


Monitorização do mosquito invasor Aedes albopictus em Portugal pela Rede de Vig...

Osório, Hugo Costa; Amaro, Fátima; Zé-Zé, Líbia; Silva, Manuel; Alves, Maria João; Equipa REVIVE

O mosquito Aedes albopictus, conhecido vulgarmente como Mos2017-2019quito Tigre Asiático, é um mosquito invasor procedente da região Ásia-Pacífico. Esta espécie é importante em saúde pública e veterinária devido ao seu papel na transmissão de diversos agentes patogénicos, nomeadamente os arbovírus chikungunya, dengue e Zika. Introduzido e estabelecido ao longo da costa mediterrânica europeia, incluindo Espanha ...


Mitogenome diversity of Aedes (Stegomyia) albopictus: Detection of multiple int...

Zé-Zé, Líbia; Borges, Vítor; Osório, Hugo Costa; Machado, Jorge; Gomes, João Paulo; Alves, Maria João

Aedes albopictus, along with Ae. aegypti, are key arbovirus vectors that have been expanding their geographic range over the last decades. In 2017, Ae. albopictus was detected for the first time at two distinct locations in Portugal. In order to understand how the Ae. albopictus populations recently introduced in Portugal are genetically related and which is their likely route of invasion, we performed an integ...


West nile virus in Portugal: vector population, host interaction and detection ...

Osório, Hugo Costa

This thesis describes the studies undertaken in the epidemiology of West Nile virus (WNV) in Portugal exploring several key components of its natural cycle: identification of mosquito vector-populations – geographical and seasonal distribution; analysis of genetic and environmental determinants on feeding patterns of mosquito vectors; experimental transmission of WNV; identification of flaviviruses detected in ...


Detection of mosquito-only flaviviruses in Europe

Calzolari, M.; Zé-Zé, Líbia; Ruzek, D.; Vazquez, A.; Jeffries, C.; Defilippo, F.; Osório, Hugo Costa; Kilian, P.; Ruíz, S.; Fooks, A.R.; Maioli, G.

The genus Flavivirus, family Flaviviridae, includes a number of important arthropod-transmitted human pathogens such as dengue viruses, West Nile virus, Japanese encephalitis virus and yellow fever virus. In addition, the genus includes flaviviruses without a known vertebrate reservoir, which have been detected only in insects, particularly in mosquitoes, such as cell fusing agent virus, Kamiti River virus, Cul...


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