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The Dystrophin-Dystroglycan complex ensures cytokinesis efficiency in Drosophil...
Gonçalves, M; Lopes, C; Alégot, H; Osswald, M; Bosveld, F; Ramos, C; Richard, G; Bellaiche, Y; Mirouse, V; Morais-de-Sá, ECytokinesis physically separates daughter cells at the end of cell division. This step is particularly challenging for epithelial cells, which are connected to their neighbors and to the extracellular matrix by transmembrane protein complexes. To systematically evaluate the impact of the cell adhesion machinery on epithelial cytokinesis efficiency, we performed an RNAi-based modifier screen in the Drosophila fo...
Systematic analysis of RhoGEF/GAP localizations uncovers regulators of mechanos...
Pietro, F; Osswald, M; Heras, JM; Cristo, I; López-Gay, J; Wang, Z; Pelletier, S; Gaugué, I; Leroy, A; Martin, C; Morais-de-Sá, E; Bellaïche YCell proliferation is central to epithelial tissue development, repair, and homeostasis. During cell division, small RhoGTPases control both actomyosin dynamics and cell-cell junction remodeling to faithfully segregate the genome while maintaining tissue polarity and integrity. To decipher the mechanisms of RhoGTPase spatiotemporal regulation during epithelial cell division, we generated a transgenic fluorescen...
aPKC regulates apical constriction to prevent tissue rupture in the Drosophila ...
Osswald, M; Barros-Carvalho, A; Carmo, AM; Loyer, N; Gracio, PC; Sunkel, CE; Homem, CCF; Januschke, J; Morais-de-Sá, EApical-basal polarity is an essential epithelial trait controlled by the evolutionarily conserved PAR-aPKC polarity network. Dysregulation of polarity proteins disrupts tissue organization during development and in disease, but the underlying mechanisms are unclear due to the broad implications of polarity loss. Here, we uncover how Drosophila aPKC maintains epithelial architecture by directly observing tissue ...
Light-induced protein clustering for optogenetic interference and protein inter...
Osswald, M; Santos, AF; Morais-de-Sá, EDrosophila Schneider 2 (S2) cells are a simple and powerful system commonly used in cell biology because they are well suited for high resolution microscopy and RNAi-mediated depletion. However, understanding dynamic processes, such as cell division, also requires methodology to interfere with protein function with high spatiotemporal control. In this research study, we report the adaptation of an optogenetic t...
PP1-Mediated Dephosphorylation of Lgl Controls Apical-basal Polarity
Moreira, S; Osswald, M; Ventura, G; Gonçalves, M; Sunkel, CE; Morais-de-Sá, EApical-basal polarity is a common trait that underlies epithelial function. Although the asymmetric distribution of cortical polarity proteins works in a functioning equilibrium, it also retains plasticity to accommodate cell division, during which the basolateral determinant Lgl is released from the cortex. Here, we investigated how Lgl restores its cortical localization to maintain the integrity of dividing e...
Protein Phosphatase 1 inactivates Mps1 to ensure efficient Spindle Assembly Che...
Moura, M; Osswald, M; Leça, N; Barbosa, J; Pereira, AJ; Maiato, H; Sunkel, CE; Conde, CFaithfull genome partitioning during cell division relies on the Spindle Assembly Checkpoint (SAC), a conserved signaling pathway that delays anaphase onset until all chromosomes are attached to spindle microtubules. Mps1 kinase is an upstream SAC regulator that promotes the assembly of an anaphase inhibitor through a sequential multi-target phosphorylation cascade. Thus, the SAC is highly responsive to Mps1, w...