41 documents found, page 1 of 5
Leaf anatomy micromorphometry plasticity and histochemistry of Azadirachta indi...
Rodrigues,Marcelo; Rocha,Diego Ismael; Mendonça,Ane Marcela das Chagas; Silva,Luzimar Campos da; Festucci-Buselli,Reginaldo Alves; Otoni,Wagner CamposAbstract Environmental conditions of grow can modify leaf structure and metabolite production. Neem plants produce a high amount of medicinal metabolites and contain biopesticide terpenoids with low toxicity. However, the high genetic variation and the low material quality, besides the environmental modifications warn to the need of biotechnological techniques to ensure the production of high quality metabolite...
Advances in passion fruit (Passiflora spp.) propagation
Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Junghans,Tatiana Góes; Jesus,Onildo Nunes de; Miranda,Diego; Otoni,Wagner CamposAbstract The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods...
Effect of Inoculation of Pineapple Plantlets with Arbuscular Mycorrhizal Fungi ...
Moreira,Bruno Coutinho; Prates Junior,Paulo; Jordão,Thuany Cerqueira; Silva,Marliane de Cássia Soares da; Ribeiro,Ana Paula FerreiraABSTRACT The production of inoculum from arbuscular mycorrhizal fungi (AMF) at a large scale and low cost is essential for establishing methods to assist in producing pineapple plantlets with high nutritional and phytosanitary quality. However, this objective is difficult to accomplish because of the biotrophic nature of these fungi. The on-farm multiplication method for AMF inoculum presents a good alternative...
AUXIN PULSE IN THE INDUCTION OF SOMATIC EMBRYOS OF Eucalyptus
Moura,Luciana Coelho de; Xavier,Aloisio; Cruz,Ana Cláudia Ferreira da; Gallo,Ricardo; Miranda,Natane Amaral; Otoni,Wagner CamposABSTRACT The objective of this study was to evaluate the effect of auxin pulse intervals on the induction of somatic embryos of Eucalyptus grandis x E. urophylla and to describe the embryogenic behavior of callus under the effect of auxinic stress. Cotyledons were inoculated in culture medium containing 207.07 µM picloram, a treatment considered as auxin pulse. Explants that were in the auxin pulse treatment we...
LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES
Souza,Denys Matheus Santana Costa; Xavier,Aloisio; Otoni,Wagner Campos; Miranda,Natane Amaral; Maggioni,Joane HelenaABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This ...
Flask sealing on in vitro seed germination and morphogenesis of two types of or...
Batista,Diego Silva; Dias,Leonardo Lucas Carnevalli; Rêgo,Mailson Monteiro do; Saldanha,Cleber Witt; Otoni,Wagner CamposABSTRACT: The influence of flask sealing and explant source on the in vitro morphogenesis of pepper, were evaluated in Capsicum annuum. Seeds were sterilized and inoculated in Murashige and Skoog media supplemented with vitamins, myo-inositol, sucrose, and agar. Gas exchange was evaluated in the germination stage by comparing 3 flask-sealing systems: rigid polypropylene lids (PLs) without vents, PLs with 1 vent...
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants
Picolotto,Dayana Rotili Nunes; Paiva Neto,Vespasiano Borges de; Barros,Fábio de; Padilha,Daly Roxana Castro; Cruz,Ana Cláudia Ferreira daAbstract An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicating phytoregulators synergistic effect. Medium supplemen...
ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill H...
Oliveira,Leandro Silva; Xavier,Aloisio; Otoni,Wagner Campos; Campos,José Marcello Salabert; Viccini,Lyderson Facio; Takahashi,Elizabete KeikoABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation ...
EFFECTS OF EXPLANT TYPE, CULTURE MEDIA AND PICLORAM AND DICAMBA GROWTH REGULATO...
Moura,Luciana Coelho de; Xavier,Aloisio; Cruz,Ana Claudia Ferreira da; Gallo,Ricardo; Gatti,Kellen Cristina; Miranda,Natane Amaral; Otoni,Wagner CamposABSTRACT The objective of the present study was to test the effects of explant type, auxin concentrations, culture media, and auxin concentrations on the induction and proliferation of somatic embryos of Eucalyptus grandis x E. urophylla. Seeds and cotyledons were used as explants and inoculated in culture media containing 1.13, 2.26, 3.39 and 4.52 µM dicamba or 4.14, 10.35, 20.71 and 31.06 µM picloram. Embryog...
IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBR...
Gallo,Ricardo; Xavier,Aloisio; Moura,Luciana Coelho de; Oliveira,Brener de Almeida; Nascimento,Heloisa Rocha do; Otoni,Wagner CamposABSTRACT This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucal...