13 documents found, page 1 of 2
Bacterial ecology of artisanal Minas cheeses assessed by culture-dependent and ...
Nero, Luís Augusto; Perin, Luana Martins; Sardaro, Maria Luisa Savo; Neviani, Erasmo; Gatti, MonicaArtisanal Minas cheese is produced in Minas Gerais state, Brazil and its varieties are named according to their geographical origin (Serro, Canastra, Serra do Salitre, Araxá and Campo das Vertentes). The cheese is produced with raw cow's milk and the whey from the previous cheese production (“pingo”). The high economic and cultural importance of artisanal cheese in Brazil justifies the efforts to ensure its saf...
Microbiota of Minas cheese as influenced by the nisin producer Lactococcus lact...
Perin, Luana Martins; Bello, Barbara Dal; Belviso, Simona; Zeppa, Giuseppe; Carvalho, Antônio Fernandes de; Cocolin, Luca; Nero, Luís AugustoMinas cheese is a popular dairy product in Brazil that is traditionally produced using raw or pasteurized cow milk. This study proposed an alternative production of Minas cheese using raw goat milk added of a nisin producer Lactococcus lactis subsp. lactis GLc05. An in situ investigation was carried on to evaluate the interactions between the L. lactis subsp. lactis GLc05 and the autochthonous microbiota of a M...
Lantibiotics biosynthesis genes and bacteriocinogenic activity of Lactobacillus...
Perin, Luana Martins; Moraes, Paula Mendonça; Silva Jr., Abelardo; Nero, Luís AugustoLactobacillus species are usually used as starters for the production of fermented products, and some strains are capable of producing antimicrobial substances, such as bacteriocins. Because these characteristics are highly desirable, research are continually being performed for novel Lactobacillus strains with bacteriocinogenic potential for use by food industries. The aim of this study was to characterise the...
Advancement in LH-PCR methodology for multiple microbial species detections in ...
Sardaro, Maria Luisa Savo; Perin, Luana Martins; Bancalari, Elena; Neviani, Erasmo; Gatti, MonicaThe length-heterogeneity PCR is a low throughput molecular biology methods explored to monitor bacteria populations in different environments. It could be more used in food microbiology analysis, not only for fingerprinting analysis, but it has been hampered until now by a limiting factor which relates to the high percentage of secondary peaks. With the aim to overcome this problem, different experiments were p...
Investigation of genes involved in nisin production in Enterococcus spp. strain...
Perin, Luana Martins; Todorov, Svetoslav Dimitrov; Nero, Luís AugustoDifferent strains of Lactococcus lactis are capable of producing the bacteriocin nisin. However, genetic transfer mechanisms allow the natural occurrence of genes involved in nisin production in members of other bacterial genera, such as Enterococcus spp. In a previous study, nisA was identified in eight enterococci capable of producing antimicrobial substances. The aim of this study was to verify the presence ...
Virulence, antibiotic resistance and biogenic amines of bacteriocinogenic lacto...
Perin, Luana Martins; Miranda, Rodrigo Otávio; Todorov, Svetoslav Dimitrov; Franco, Bernadette Dora Gombossy de Melo; Nero, Luís AugustoThe present study aimed to investigate the virulence, antibiotic resistance and biogenic amine production in bacteriocinogenic lactococci and enterococci isolated from goat milk in order to evaluate their safety. Twenty-nine bacteriocinogenic lactic acid bacteria (LAB: 11 Lactococcus spp., and 18 Enterococcus spp.) isolated from raw goat milk were selected and subjected to PCR to identify gelE, cylA, hyl, asa1,...
Genetic diversity and some aspects of antimicrobial activity of lactic acid bac...
Cavicchioli, Valéria Quintana; Dornellas, Wesley dos Santos; Perin, Luana Martins; Pieri, Fábio Alessandro; Franco, Bernadette Dora Gombossy de MeloLactic acid bacteria (LAB, n = 57) were previously obtained from raw goat milk, identified as Lactococcus spp. (n = 24) and Enterococcus spp. (n = 33), and characterized as bacteriocinogenic. Fingerprinting by pulsed field gel electrophoresis (PFGE) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (LAB, spoilage, and foodborn...
Comparison of phenotypic and molecular tests to identify lactic acid bacteria
Moraes, Paula Mendonça; Perin, Luana Martins; Silva Júnior, Abelardo; Nero, Luís AugustoTwenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
Antagonistic lactic acid bacteria isolated from goat milk and identification of...
Perin, Luana Martins; Nero, Luís AugustoThe raw goat milk microbiota is considered a good source of novel bacteriocinogenic lactic acid bacteria (LAB) strains that can be exploited as an alternative for use as biopreservatives in foods. The constant demand for such alternative tools justifies studies that investigate the antimicrobial potential of such strains. The obtained data identified a predominance of Lactococcus and Enterococcus strains in raw...
Glucuronidase activity of escherichia coli isolated from chicken carcasses
Perin, Luana Martins; Yamazi, Anderson Keizo; Moraes, Paula Mendonça; Cossi, Marcus Vinícius Coutinho; Pinto, Paulo Sérgio de Arruda; Nero, Luís AugustoTo identify Escherichia coli through the production of -D-glucuronidase (GUD), 622 suspect cultures were isolated from chicken carcasses and plated in PetrifilmTM EC. Of these cultures, only 44 (7.1%) failed to produce GUD. This result indicates the usefulness of GUD production for estimating E. coli populations in chicken.