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Exploring the Potential of Olive By-Products in Bísaro Pig Feed: Effects on the...

Paié-Ribeiro, Jessica; Pinheiro, Victor; Guedes, Cristina; Gomes, Maria José; Teixeira, José; Leite, Ana; Vasconcelos, Lia; Teixeira, Alfredo

The olive oil industry generates large quantities of olive cake (OC), making its use in animal feed an environmentally sustainable alternative. Considering that the ham of Bísaro pigs is traditionally used for the production of cured ham, the analysis of raw meat is essential to understand how diet influences its quality before the maturation process. This study examined the effect of different types of OC in t...

Date: 2025   |   Origin: Biblioteca Digital do IPB

Selection of aptamers to target enterotoxigenic Escherichia coli infections

Campos, Ana Maria; Barros, Maria Margarida; Oliveira, Ricardo; Castro, Joana Isabel Reis; Araújo, Daniela Eira; Outor-Monteiro, Divanildo

[Excerpt] Introduction Enterotoxigenic Escherichia coli (ETEC) is the leading cause of enteric infections in swine, resulting in significant costs for the industry (1 ). Initial adhesion to the intestinal epithelium relies on fimbriae, with F4 and F18 types being most associated with enteric infections. The traditional use of antibiotics for prevention and treatment is increasingly restricted due to rising anti...


Swine industry: validated PCR-based method for detecting enterotoxigenic Escher...

Barros, Margarida; Castro, Joana Isabel Reis; Oliveira, Ricardo; Campos, Ana Maria; Araújo, Daniela; Silva, Sónia Carina; Almeida, Gonçalo Nieto

[Excerpt] Enterotoxigenic E. coli (ETEC) infections lead to significant costs for the swine industry worldwide. Bacterial fimbriae (F4/F5/F6/F18/F41) allow the adhesion of the bacteria to epithelial cells, and when both the immunological systems and the gut mic ro biota are poorly developed, ETEC colonizes and produces one or more enterotoxins (LT/ STa/ STb) that can have local and systemic effects. Therefore, ...


An asset for food safety: the knowledge behind the physiological alterations in...

Barros, Maria Margarida; Campos, Ana Maria; Castro, Joana Isabel Reis; Oliveira, Ricardo; Araújo, Daniela Eira; Outor-Monteiro, Divanildo

Foodborne pathogens represent a significant public health risk in both developed and developing countries. Among these pathogens, enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in humans and one of the leading causes of mortality in newly weaned pigs. The main sources of ETEC contamination include environments with poor hygiene and contaminated water, meat, cereals, and vegetables. Therefo...


From Waste to Resource: Compositional Analysis of Olive Cake’s Fatty Acids, Nut...

Paié-Ribeiro, Jessica; Baptista, Filipa; Teixeira, José; Guedes, Cristina; Gomes, Maria J.; Teixeira, Alfredo; Barros, Ana Novo; Pinheiro, Victor

The olive oil industry, recognised for its beneficial products for health and food culture, generates a significant amount of by-products that, if not appropriately managed, can pose considerable environmental challenges. This study examined six olive cakes (OC) from the Trás-os-Montes and Alto Douro regions, collected on different dates and mills: two obtained by pressing (COC), two by centrifugation (TPOC), i...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Incorporating Olive By-Products in Bísaro Pig Diets: Effect on Dry-Cured Produc...

Leite, Ana; Vasconcelos, Lia; Lopez, Sergio; Outor-Monteiro, Divanildo; Pinheiro, Victor; Rodrigues, Sandra; Teixeira, Alfredo

The objective of this study was to assess the impact of incorporating olive cake into the diet of indigenous Bísaro pigs on the quality of processed meat products. To this end, loins and “cachaços” were processed using a standardized manufacturing flowchart to produce dry-cured products. The two products were manufactured using the same formulation, ingredients, and curing process. Concerning the physicochemica...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Exploring the Variability in Phenolic Compounds and Antioxidant Capacity in Oli...

Paié-Ribeiro, Jessica; Baptista, Filipa; Gomes, Maria José Marques; Teixeira, Alfredo; Pinheiro, Victor; Outor-Monteiro, Divanildo; Barros, Ana Novo

The olive oil industry generates large volumes of by-products, creating notable environmental and economic concerns. Among these, olive cake (OC)—a primary by-product of olive oil extraction—stands out due to its high content of bioactive compounds and potential for value-added recycling. This study focused on characterizing six OC samples from the Trás-os-Montes and Alto Douro regions, collected at different p...

Date: 2024   |   Origin: Biblioteca Digital do IPB

In vivo study of the effect of sugarcane bagasse lignin supplementation on broi...

Carvalho, Nelson Mota de; Souza, Carla Giselly de; Costa, Célia Maria; Castro, Cláudia; Fangueiro, Joana F.; Horta, Bruno; Outor-Monteiro, Divanildo

Since the global restrictions on antibiotics in poultry systems, there has been a growing demand for natural and sustainable feed additives for disease prevention and poultry nutrition. This study evaluated the effects of incorporating sugarcane bagasse (SCB) lignin into broiler chicken diets. The performance of the chickens, including body weight, feed intake, and mortality, as well as intestinal histomorphome...


Application of DNA aptamers to block enterotoxigenic Escherichia coli toxicity ...

Barros, Maria Margarida; Castro, Joana Isabel Reis; Araújo, Daniela Eira; Oliveira, Ricardo; Campos, Ana Maria; Silva, Sónia Carina

Enterotoxigenic Escherichia coli (ETEC) is the major bacterial cause of diarrheal diseases in pigs, particularly at young ages, resulting in significant costs to swine farming. The pathogenicity of ETEC is largely dependent on the presence of fimbriae and the ability to produce toxins. Fimbriae are responsible for their initial adhesion to the intestinal epithelial cells, leading to the onset of infection. In p...


Detecting enterotoxigenic Escherichia coli in animal production: method develop...

Barros, Maria Margarida; Oliveira, Ricardo; Castro, Joana Isabel Reis; Campos, Ana; Almeida, Gonçalo Nieto; Araújo, Daniela; Silva, Sónia Carina

Swine enteric colibacillosis is a disease characterized by an intestinal infection caused by enterotoxigenic Escherichia coli (ETEC). This infection mostly causes illness or death in neonatal and weaned pigs making it responsible for significant economic losses. Bacterial fimbriae (F4/F5/F6/F18/F41) are responsible for the adhesion to epithelial cells, and when both the immunological systems and the gut microbi...


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