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How temperature modulates the expression of pathogenesis-related molecules of t...

Félix, Carina; Meneses, Rodrigo; Gonçalves, Micael F. M.; Duarte, Ana S.; Jorrín-Novo, Jesus V.; van de Peer, Yves; Deforce, Dieter

Lasiodiplodia hormozganensis, initially recognized as a fungal plant pathogen, is recognized now acknowledged as a potential threat to humans. However, our understanding of the pathogenesis mechanisms of Lasiodiplodia species remains limited, and the impact of temperature on its pathogenicity is unclear. This study aims to elucidate the effects of temperature on the biology of L. hormozganensis, focusing on the...


Human Gingival Fibroblasts Response to Different Endodontic Sealers: An In Vitr...

Noites, Rita; Tavares, Inês; Cardoso, Miguel; Carreira, Isabel M.; Bartolomeu, Maria; Duarte, Ana S.; Ribeiro, Ilda Patrícia

Endodontic treatment aims to eliminate infection of the root canals and fill the dental pulp space. The biocompatibility studies of the sealers used in root canals obturation are crucial since they are applied in direct contact with periradicular tissues. Objective: The aim of this study was to evaluate the cytotoxicity of three root canal sealers—AH Plus, Bio MTA+, and Bio C sealer—on immortalized human gingiv...


Multiplex immunoassay for inflammatory proteins quantification in saliva – a me...

Rosa, Nuno; Gomes, Ana T. P. C.; Mendes, Karina; Duarte, Ana S.; Silva, Raquel M. S.; Correia, Maria; Barros, Marlene


Microbial DNA extraction methods for microbial screening - saliva vs biofilm co...

Gomes, Ana T. P. C.; Pinto, Marla; Abrantes, Patrícia; Almeida, Rita; Mendes, Karina; Duarte, Ana S.; Silva, Raquel M.; Rosa, Nuno; Correia, Maria


Human gingival fibroblasts response to different endodontic sealers: an in vitr...

Noites, Rita; Tavares, Inês; Cardoso, Miguel; Carreira, Isabel M.; Duarte, Ana S.

Endodontic treatment aims to eliminate infection of the root canals and fill the dental pulp space. The biocompatibility studies of the sealers used in root canals obturation are crucial since they are applied in direct contact with periradicular tissues. Objective: The aim of this study was to evaluate the cytotoxicity of three root canal sealers—AH Plus, Bio MTA+, and Bio C sealer—on immortalized human gingiv...


New and efficient bioactive glass compositions for controlling endodontic patho...

Correia, Bruna L.; Gomes, Ana T.P.C.; Noites, Rita; Ferreira, José M. F.; Duarte, Ana S.

Endodontic treatment aims to conserve teeth through removing infected tissue, disinfecting, and filling/sealing the root canal. One of the most important treatment steps is the removal of microorganisms to avoid reinfection and consequent tooth loss. Due to increased resistance to intracanal medications, new alternative procedures are needed. Thus, an intracanal medication is suggested using three bioactive gla...


Antimicrobial activity of a 3D-printed polymethylmethacrylate dental resin enha...

Salgado, Helena; Gomes, Ana T. P. C.; Duarte, Ana S.; Ferreira, José M. F.; Fernandes, Carlos; Figueiral, Maria Helena; Mesquita, Pedro

The present study aimed to test, in vitro, the antimicrobial activity against Candida albicans and Streptococcus mutans and the surface roughness of a 3D-printed polymethylmethacrylate dental resin enhanced with graphene. A 3D-printed polymethylmethacrylate dental resin was reinforced with four different concentrations of graphene: 0.01, 0.1, 0.25 and 0.5 wt%. Neat resin was used as a control. The specimens wer...


Can corrole dimers be good photosensitizers to kill bacteria?

Lacerda, Paula S. S.; Bartolomeu, Maria; Gomes, Ana T. P. C.; Duarte, Ana S.; Almeida, Adelaide; Faustino, Maria A. F.; Neves, Maria G. P. M. S.

Corroles possess key photophysical and photochemical properties to be exploited as thera-peutic agents in antimicrobial photodynamic therapy (aPDT). Herein, we present for the first time the antimicrobial efficiency of three corrole dimers and of the corresponding precursor against the Gram(+) bacterium Staphylococcus aureus. Additionally, to explore future clinical applications, the cytotoxicity of the most pr...


Genotypic and phenotypic traits of blaCTX-M-carrying Escherichia coli strains f...

Tavares, Rafael D. S.; Tacão, Marta; Figueiredo, Ana S.; Duarte, Ana S.; Esposito, Fernanda; Lincopan, Nilton; Manaia, Célia M.; Henriques, Isabel

Wastewater treatment plants (WWTPs) are relevant sources of antibiotic resistance into aquatic environments. Disinfection of WWTPs’ effluents (e.g. by UV-C irradiation) may attenuate this problem, though some clinically relevant bacteria have been shown to survive disinfection. In this study we characterized 25 CTX-M-producing Escherichia coli strains isolated from a WWTP’s UV-C-irradiated effluent, aiming to i...


Genotypic and phenotypic traits of blaCTX-M-carrying Escherichia coli strains f...

Tavares, Rafael D. S.; Tacão, Marta; Figueiredo, Ana S.; Duarte, Ana S.; Esposito, Fernanda; Lincopan, Nilton; Manaia, Célia M.; Henriques, Isabel

Wastewater treatment plants (WWTPs) are relevant sources of antibiotic resistance into aquatic environments. Disinfection of WWTPs' effluents (e.g. by UV-C irradiation) may attenuate this problem, though some clinically relevant bacteria have been shown to survive disinfection. In this study we characterized 25 CTX-M-producing Escherichia coli strains isolated from a WWTP's UV-C-irradiated effluent, aiming to i...


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