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Portuguese cork industry: filling the knowledge gap regarding occupational expo...

Viegas, Carla; Dias, Marta; Pacífico, Cátia; Faria, Tiago; Clérigo, Anália Matos; Dias, Hermínia Brites; Caetano, Liliana Aranha; Carolino, Elisabete

Introduction: The presence of the Penicillium section Aspergilloides (formerly known as Penicillium glabrum) in the cork industry involves the risk of respiratory diseases such as suberosis. Methods: This study aimed to corroborate the predominant fungi presents in this occupational environment by performing a mycological analysis of 360 workers’ nasal exudates collected by nasal swabs. Additionally, evaluation...


Portuguese cork industry: filling the knowledge gap regarding occupational expo...

Viegas, Carla; Dias, Marta; Pacífico, Cátia; Faria, Tiago; Clérigo, Anália; Brites, Hermínia; Caetano, Liliana Aranha; Carolino, Elisabete

Introduction: The presence of the Penicillium section Aspergilloides (formerly known as Penicillium glabrum) in the cork industry involves the risk of respiratory diseases such as suberosis. Methods: The aim of this study was to corroborate the predominant fungi present in this occupational environment by performing a mycological analysis of 360 workers' nasal exudates collected by nasal swabs. Additionally, ev...


Nasal swab as a tool to access occupational exposure to fungi in a cork industry

Viegas, Carla; Pacífico, Cátia; Faria, Tiago; Aranha Caetano, Liliana; Gomes, Anita Quintal; Viegas, Susana

Approximately 49% of all cork produced worldwide in 2016 was from Portugal, where there are 650 companies working in this production sector. Additionally, two thirds of the world exportation comes from Portugal, with Portuguese cork industry employing about ten thousands workers (MTSS 2009). The presence of the Penicillium section Aspergilloides (formerly known as Penicillium glabrum) in this industry involves ...


Natural occurrence of Escherichia coli-infecting bacteriophages in clinical sam...

Pacífico, Cátia; Hilbert, Miriam; Sofka, Dmitrij; Dinhopl, Nora; Pap, Ildiko-Julia; Aspöck, Christoph; Carrico, Joao Andre; Hilbert, Friederike

The interaction between bacteriophages, bacteria and the human host as a tripartite system has recently captured attention. The taxonomic diversity of bacteriophages, as a natural parasite of bacteria, still remains obscure in human body biomes, representing a so-called "viral dark matter." Here, we isolated and characterized coliphages from blood, urine and tracheal aspirates samples collected at a tertiary ca...


Fungal contamination in green coffee beans samples: a public health concern

Viegas, Carla; Pacífico, Cátia; Faria, Tiago; Oliveira, Ana Cebola; Caetano, Liliana Aranha; Carolino, Elisabete; Gomes, Anita Quintal; Viegas, Susana

Studies on the microbiology of coffee cherries and beans have shown that the predominant toxigenic fungal genera (Aspergillus and Penicillium) are natural coffee contaminants. The aim of this study was to investigate the distribution of fungi in Coffea arabica L. (Arabica coffee) and Coffea canephora L. var. robusta (Robusta coffee) green coffee samples obtained from different sources at the pre-roasting stage....


Microbiota and particulate matter assessment in Portuguese optical shops provid...

Viegas, Carla; Faria, Tiago; Pacífico, Cátia; Santos, Mateus dos; Monteiro, Ana; Lança, Carla; Carolino, Elisabete; Viegas, Susana; Cabo-Verde, Sandra

The aim of this work was to assess the microbiota (fungi and bacteria) and particulate matter in optical shops, contributing to a specific protocol to ensure a proper assessment. Air samples were collected through an impaction method. Surface and equipment swab samples were also collected side-by-side. Measurements of particulate matter were performed using portable direct-reading equipment. A walkthrough surve...


Cytotoxic and inflammatory potential of air samples from occupational settings ...

Viegas, Susana; Aranha Caetano, Liliana; Korkalainen, Merja; Faria, Tiago; Pacífico, Cátia; Carolino, Elisabete; Gomes, Anita Quintal; Viegas, Carla

Organic dust and related microbial exposures are the main inducers of several respiratory symptoms. Occupational exposure to organic dust is very common and has been reported in diverse settings. In vitro tests using relevant cell cultures can be very useful for characterizing the toxicity of complex mixtures present in the air of occupational environments such as organic dust. In this study, the cell viability...


Fungal contamination in green coffee beans samples: a public health concern

Viegas, Carla; Pacífico, Cátia; Faria, Tiago; de Oliveira, Ana Cebola; Caetano, Liliana Aranha; Carolino, Elisabete; Gomes, Anita Q.; Viegas, Susana

Studies on the microbiology of coffee cherries and beans have shown that the predominant toxigenic fungal genera (Aspergillus and Penicillium) are natural coffee contaminants. The aim of this study was to investigate the distribution of fungi in Coffea arabica L. (Arabica coffee) and Coffea canephora L. var. robusta (Robusta coffee) green coffee samples obtained from different sources at the pre-roasting stage....


Cytotoxic and inflammatory potential of air samples from occupational settings ...

Viegas, Susana; Caetano, Liliana Aranha; Korkalainen, Merja; Faria, Tiago; Pacífico, Cátia; Carolino, Elisabete; Gomes, Anita Q.; Viegas, Carla

Organic dust and related microbial exposures are the main inducers of several respiratory symptoms. Occupational exposure to organic dust is very common and has been reported in diverse settings. In vitro tests using relevant cell cultures can be very useful for characterizing the toxicity of complex mixtures present in the air of occupational environments such as organic dust. In this study, the cell viability...


New approach to study the real exposure to fungi in cork industry: nasal swabs ...

Viegas, Carla; Clérigo, Anália; Pacífico, Cátia; Faria, Tiago; Sabino, Raquel; Francisco, Mariana; Veríssimo, Cristina; Viegas, Susana

The permanent contact with cork may lead to constant exposure to fungi, raising awareness as a potential occupational hazard in the cork industry.The presence of fungi belonging to the Penicillium glabrum complex has been associated with the development of respiratory diseases such as suberosis, one of the most prevalent diseases among workers from cork industries, besides occupational asthma. Azoles are used a...


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