42 documents found, page 1 of 5
Looking at the potential of marine macroalgae supplementation to afford neuropr...
Neto, Ana; Brandão, Fátima; Almeida, Armando; Abreu, Helena; Pousão-Ferreira, Pedro; Cesário, Rute; Pacheco, Mário; Pereira, PatríciaBackground: Marine macroalgae have potential as a source of many natural compounds with health benefits [1], but their use to mitigate aquatic contaminants bioaccumulation in fish and resultant toxicity is an underexplored research topic. Objective: Asses if a marine macroalgae-enriched diet can provide neuroprotection Diplodus sargus when exposed to waterborne inorganic mercury (iHg), namely by reducing bioacc...
Integrated Use of Bioaccumulation, Genotoxic, and Haematological Endpoints to A...
Mieiro, Cláudia Leopoldina; Coelho, João; Reis, Ana; Castro, Diana; Figueira, Paula; Martinho, Filipe; Pardal, Miguel; Pereira, Eduarda; Pacheco, MárioBiosorption successfully remediates saline water contaminated with legacy contaminants, but its effects on the health of marine organisms remain unclear. Therefore, our aim was to address this knowledge gap with data on the accumulation ability, as well as the cytogenetic and biochemical effects in turbot (Scophthalmus maximus). To this end, we exposed turbot for seven days to a mixture of remediated metals (Re...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to humans. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers, some chemically induced DNA adducts. Depending on the specimen type, there ar...
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...
Integrated Use of Bioaccumulation, Genotoxic, and Haematological Endpoints to A...
Mieiro, Cláudia; Coelho, João; Reis, Ana Teresa; Castro, Diana; Figueira, Paula; Martinho, Filipe; Pardal, Miguel; Pereira, Eduarda; Pacheco, MárioBiosorption successfully remediates saline water contaminated with legacy contaminants, but its effects on the health of marine organisms remain unclear. Therefore, our aim was to address this knowledge gap with data on the accumulation ability, as well as the cytogenetic and biochemical effects in turbot (Scophthalmus maximus). To this end, we exposed turbot for seven days to a mixture of remediated metals (Re...
Comparative neurotoxicity of dietary methylmercury and waterborne inorganic mer...
Carvalho, Patrícia S.; Fonseca-Rodrigues, Diana; Pacheco, Mário; Almeida, Armando; Pinto-Ribeiro, Filipa; Pereira, PatríciaThis work investigated the effects of inorganic mercury (iHg) and methylmercury (MeHg) on the fish optic tectum morphology, viz. in relation to: i) vulnerability of specific optic tectum layers; ii) preferential targeting of Hg forms to neurons or glial cells; iii) comparative toxicity of iHg and MeHg in this brain area that is in the maintenance of several fish behaviors. Two experiments exposing juvenile whit...
Red seaweeds strengthening the nexus between nutrition and health: phytochemica...
Ferreira, João; Hartmann, Anja; Martins-Gomes, Carlos; Nunes, Fernando M.; Souto, Eliana B.; Santos, Dario L.; Abreu, Helena; Pereira, RuiThe red seaweeds Grateloupia turuturu and Porphyra umbilicalis are commonly used as human food, mainly in East Asia, being valuable nutritional sources. However, studies focusing on their composition and bioactivities are scarce. Thus, G. turuturu and P. umbilicalis hydroethanolic and water (infusion and decoction) extracts were prepared to characterize their bioactive compounds; assess antioxidant activity aga...
DNA damage and oxidative stress responses of mussels Mytilus galloprovincialis ...
Braga, Ana C.; Pereira, Vitória; Marçal, Raquel; Marques, Ana; Guilherme, Sofia; Costa, Pedro R.; Pacheco, MárioCommonly affected by changes in climate and environmental conditions, coastal areas are very dynamic environments where shellfish play an important ecological role. In this study, the oxidative stress and genotoxic responses of mussels (Mytilus galloprovincialis) exposed to paralytic shellfish toxin (PST) - producing dinoflagellates Gymnodinium catenatum were evaluated under i) current conditions (CC: 19 °C; pH...
A multidimensional concept for mercury neuronal and sensory toxicity in fish - ...
Pereira, Patrícia; Korbas, Malgorzata; Pereira, Vitória; Cappello, Tiziana; Maisano, Maria; Canário, João; Almeida, Armando; Pacheco, MárioNeuronal and sensory toxicity of mercury (Hg) compounds has been largely investigated in humans/mammals with a focus on public health, while research in fish is less prolific and dispersed by different species. Well-established premises for mammals have been governing fish research, but some contradictory findings suggest that knowledge translation between these animal groups needs prudence [e.g. the relative h...
Marine macroalgae as a dietary source of genoprotection in gilthead seabream (S...
Pereira, Vitória; Marques, Ana; Gaivão, Isabel; Rego, Andreia; Abreu, Helena; Pereira, Rui; Santos, Maria Ana; Guilherme, Sofia; Pacheco, MárioDNA integrity and stability are essential to organisms' health and survival. However, it has been neglected in what concerns to fish farming, disregarding the potential impact of endogenous/ exogenous factors. As marine macroalgae constitute a source of natural compounds with a large spectrum of biological activities, this study, situated in the interface of nutritional-genetic research and development of algae...