5 documents found, page 1 of 1
Measuring DNA modifications with the comet assay: a compendium of protocols
Collins, Andrew; Moller, Peter; Gajski, Goran; Vodenkova, Sona; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, StefanoThe comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there ...
Invasive clams (Ruditapes philippinarum) are better equipped to deal with harmf...
Braga, Ana C.; Marcal, Raquel; Marques, Ana; Guilherme, Sofia; Vilarino, Oscar; Martins, J. Manuel Leao; Gago-Martinez, Ana; Reis Costa, PedroThis study aims to assess and compare the kinetics (accumulation/elimination) of the marine biotoxins okadaic acid (OA) and dinophysistoxin-1 (DTX1 ), between native (Ruditapes decussates) and invasive (Ruditapes philippinarum) clam species, and their genotoxic effects and DNA recover capacity after, exposure to toxic dinoflagellates Promcentrum lima. Clams were fed with P. lima for 5 days and then to non-toxic...
DNA damage and oxidative stress responses of mussels Mytilus galloprovincialis ...
Braga, Ana C.; Pereira, Vitoria; Marcal, Raquel; Marques, Ana; Guilherme, Sofia; Reis Costa, Pedro; Pacheco, MarioCommonly affected by changes in climate and environmental conditions, coastal areas are very dynamic environments where shellfish play an important ecological role. In this study, the oxidative stress and genotoxic responses of mussels (Mytilus galloprovincialis) exposed to paralytic shellfish toxin (PST) - producing dinoflagellates Gymnodinium catenatum were evaluated under i) current conditions (CC: 19 degree...
Native (Ruditapes decussatus) and non-indigenous (R. philippinarum) shellfish s...
Braga, Ana C.; Lage, Sandra; Pacheco, Mario; Rydberg, Sara; Reis Costa, PedroThe native Ruditapes decussatus and the non-indigenous Ruditapes philippinarum are an important target of shellfish industries. The aim of this study was to compare an invader with a native species living in sympatry in the view of marine biotoxins accumulation. Samples were analysed for regulated and non regulated biotoxins. The consistently occurrence of okadaic acid-group toxins and BMAA, may cause human hea...
Morphological, compositional and ultrastructural changes in the Scrobicularia p...
Ahmad, Iqbal; Singh, Manoj K.; Pereira, Maria L.; Pacheco, Mario; Santos, Maria A.; Duarte, Armando C.; Pereira, Eduarda; Gracio, JoseThe study aimed to assess morphological, structural and compositional alterations in Scrobicularia plana nacre environmentally exposed to mercury in order to seek out the possibility of the assessed alterations as a monitoring tool to handle complexity and interactions of metals in the environment involving a non-invasive methodology. Bivalves were collected from a mercury contaminated site (Laranjo basin - Ria...