105 documents found, page 1 of 11
Design of a lipid nano-delivery system containing recombinant Candida albicans ...
Costa-Barbosa, Augusto; Pacheco, Maria Inês; Carneiro, Catarina; Botelho, Cláudia M.; Gomes, Andreia C; Real Oliveira, M. Elisabete C.D.; Collins, TonyOpportunistic fungi cause lethal systemic infections and impose high medical costs to health systems. The World Health Organization has recognized the importance of fungal infections, including them in its global priority list guiding research, development, and discovery of new therapeutic approaches. Fungal vaccine development has been proposed as one of the treatment and prevention strategies in the last deca...
Whole-genome sequencing and annotation of the yeast Clavispora santaluciae reve...
Franco-Duarte, Ricardo; Čadež, Neža; Rito, Teresa; Drumonde-Neves, João; Dominguez, Yazmid Reyes; Pais, Célia; Sousa, Maria João; Soares, Pedro<i>Clavispora santaluciae</i> was recently described as a novel non-<i>Saccharomyces</i> yeast species, isolated from grapes of Azores vineyards, a Portuguese archipelago with particular environmental conditions, and from Italian grapes infected with <i>Drosophila suzukii</i>. In the present work, the genome of five <i>Clavispora santaluciae</i> strains was sequenced, assembled, and annotated for the first time...
Development and Characterization of Monoolein-Based Liposomes of Carvacrol, Cin...
Miranda-Cadena, Katherine; Dias, Marisol; Costa-Barbosa, Augusto; Collins, Tony; Marcos-Arias, Cristina; Eraso, Elena; Pais, Célia; Quindos, GuillermoVolume 65, no. 4, e01628-20, 2021, https://doi.org/10.1128/AAC.01628-20. In the original published version of this paper, posted on 18 March 2021, incorrect files were used for Fig. 6 to 10. In addition, “P, 0.5” should be “P, 0.05” in the legends of Fig. 7, 8, and 10, and in the 5th paragraph of the Results section, “64” in the 2nd-to-last sentence should be “32” and “P, 0.5“in the last sentence should be “P, ...
Development and characterization of monoolein-based liposomes of carvacrol, cin...
Miranda-Cadena, Katherine; Dias, Marisol; Costa-Barbosa, Augusto; Collins, Tony; Marcos-Arias, Cristina; Eraso, Elena; Pais, Célia; Quindos, GuillermoThere is an increasing need for novel drugs and new strategies for the therapy of invasive candidiasis. This study aimed to develop and characterize liposome-based nanoparticles of carvacrol, cinnamaldehyde, citral, and thymol with anti-Candida activities. Dioctadecyldimethylammonium bromide- and monoolein-based liposomes in a 1:2 molar ratio were prepared using a lipid-film hydration method. Liposomes were ass...
Improvement of Torulaspora delbrueckii genome annotation: towards the exploitat...
Santiago, Carolina; Rito, Teresa S; Vieira, Daniel; Fernandes, Ticiana; Pais, Célia; Sousa, Maria João; Soares, Pedro; Franco-Duarte, RicardoSaccharomyces cerevisiae is the most commonly used yeast in wine, beer, and bread fermentations. However, Torulaspora delbrueckii has attracted interest in recent years due to its properties, ranging from its ability to produce flavor- and aroma-enhanced wine to its ability to survive longer in frozen dough. In this work, publicly available genomes of T. delbrueckii were explored and their annotation was improv...
Oral Candida albicans colonization in healthy individuals: prevalence, genotypi...
Gerós-Mesquita, Ângela; Carvalho-Pereira, Joana; Franco-Duarte, Ricardo; Alves, Armandino; Gerós, H.; Pais, Célia; Sampaio, PaulaIn this study, 181 healthy individuals, including 29 couples, were analysed regarding oral yeast colonization using a culture-based approach. Results showed that 39% of the individuals were yeast carriers, 89% being colonized with Candida albicans, 5% with C. guilliermondi, 3% with C. lusitaniae and 3% with C. parapsilosis. Sixty-two percent of the couples had at least one member colonized. Colonization and CFU...
Towards a circular bioeconomy. Volatile fatty acid platform for biowaste recycling
Michels, Jochen; Mocking, Tijs; Sellis, Ido; Meerburg, Francis; Bauer, Csaba; Pais, Célia; Magielse, Peter; Ferreira, Vasco; Gonzalez, ElisabethResources in general are not infinitely available, and also renewable resources if consumed outside their normal replacement cycles become scarce. Therefore, the establishment of a circular bioeconomy must respect natural systems and replacement cycles of organic carbon thereby reducing environmental pressure of human consumption. Upcycling of side and biowaste streams towards added value compounds represents h...
Single cell oil production by oleaginous yeasts grown in synthetic and waste-de...
Bettencourt, Sara; Miranda, Catarina; Pozdniakova, Tatiana A.; Sampaio, Paula; Franco-Duarte, Ricardo; Pais, CéliaFour yeast isolates from the species—<i>Apiotrichum brassicae</i>, <i>Candida tropicalis</i>, <i>Metschnikowia pulcherrima</i>, and <i>Pichia kudriavzevii</i>—previously selected by their oleaginous character and growth flexibility in different carbon sources, were tested for their capacity to convert volatile fatty acids into lipids, in the form of single cell oils. Growth, lipid yields, volatile fatty acids c...
Multiplex PCR based strategy for detection of fungal pathogen DNA in patients w...
Carvalho-Pereira, Joana; Fernandes, Filipa; Araújo, Ricardo; Springer, Jan; Loeffler, Juergen; Buitrago, María José; Pais, Célia; Sampaio, PaulaA new and easy polymerase chain reaction (PCR) multiplex strategy, for the identification of the most common fungal species involved in invasive fungal infections (IFI) was developed in this work. Two panels with species-specific markers were designed, the <i>Candida Panel</i> for the identification of <i>Candida</i> species, and the <i>Filamentous Fungi Panel</i> for the identification of <i>Aspergillus</i> sp...
Modified high-throughput Nile red fluorescence assay for the rapid screening of...
Miranda, Catarina; Bettencourt, Sara; Pozdniakova, T. A.; Pereira, Joana; Sampaio, Paula; Franco-Duarte, Ricardo; Pais, CéliaBackground: Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use of alternative and ecological carbon sources for yeast growth. Results: In the present study a...