83 documents found, page 1 of 9
Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symport...
Barata-Antunes, Cláudia; Warmerdam, Marieke; Hulster, Erik de; Ribeiro, Inês P.; Cardoso, Clara; Leite, Beatriz; Casal, Margarida; Gerós, H.Background The Saccharomyces cerevisiae Jen1 transporter mediates electroneutral proton symport of lactate and pyruvate. In lactate-grown batch cultures, growth-coupled medium alkalinization was previously shown to coincide with endocytosis of Jen1. Results To investigate the physiological relevance of pH-dependent Jen1 endocytosis, S. cerevisiae was grown in carbon-limited continuous cultures on a mixed ethano...
Expansion, functional diversification, and gene fusion events in the Ato protei...
Ghasemi, Faezeh; Ataíde, Patrícia; Barata-Antunes, Cláudia; Pyrris, Yiannis; Alves, João; Fernandes, Vitor; Alves, Rosana; Gomes-Gonçalves, AlexandraCandida albicans, a commensal opportunistic pathogen, exhibits remarkable metabolic flexibility and adaptability to environmental changes. In glucose-limited niches, it utilizes alternative carbon sources such as carboxylic acids, which may influence its pathogenicity. In Saccharomyces cerevisiae, the uptake of monocarboxylates occurs through regulated plasma membrane (PM) transport proteins, such as Ato1 (Ady2...
Lactic acid influences iron assimilation by a fungal pathogen via the iron redu...
Gomes-Gonçalves, Alexandra; Van Genechten, Wouter; Ataíde, Patrícia; Barata-Antunes, Cláudia; Ghasemi, Faezeh; Casal, Margarida; Teixeira, Miguel C.Candida albicans is a fungal commensal of humans that often causes mucosal infections in otherwise healthy individuals and also serious infections in immunocompromised patients. The capacity of this fungus to colonize and cause disease relies on its ability to grow within the host, adapting to various nutrient restrictions and physicochemical conditions. The presence of alternative carbon sources, such as the l...
The <i>ATO</i> gene family governs <i>Candida albicans</i> colonization in the ...
Alves, Rosana; Ghasemi, Faezeh; Van Genechten, Wouter; Wijnants, Stefanie; Van Goethem, Odessa; Barata-Antunes, Cláudia; Fernandes, VitorThe fungal pathogen Candida albicans colonizes the human gut, where short-chain fatty acids (SCFAs) serve as a source of carbon. This fungus harbors one of the largest microbial families of ATO (acetate transporter ortholog) genes, which encode putative SCFA transport proteins. Here, we generate C. albicans null mutants lacking individual or all known putative SCFA transporter genes and compare their phenotypes...
From Bariatric Surgery to Conception: The Ideal Timing to Optimize Fetal Weight
Carreira, Ana; Araújo, Bárbara; Lavrador, Mariana; Vieira, Inês; Rodrigues, Dírcea Maria Trigo; Paiva, Sandra; Melo, Miguel; Paiva, IsabelPurpose Bariatric surgery (BS) increases the risk of small for gestational age (SGA) neonates. Guidelines recommend postponing pregnancy for 12–24 months, but optimal surgery-to-conception interval (BSCI) remains uncertain. We aimed to evaluate the impact of BSCI on birth weight and SGA. Materials and Methods Retrospective cohort study of 42 pregnancies following BS, including Roux-en-Y gastric bypass, gastric ...
Association between the number of altered late potential criteria and increased...
Brito, Joana; Cortez-Dias, Nuno; Lima Da Silva, Gustavo; Ferreira, Afonso Nunes; Aguiar-Ricardo, Inês; Cunha, Nelson; António, Pedro SilvérioBackground: Brugada syndrome (BrS) is associated with abnormal electrophysiological properties at right ventricular epicardium, consisting of fragmented electrograms extending well beyond QRS termination. We aimed to evaluate the utility of signal-averaged electrocardiogram (SA-ECG) for the noninvasive assessment of late potentials (LP) and risk stratification of BrS patients. Methods: A prospective, observatio...
Abnormal Responses in Cognitive Impulsivity Circuits Are Associated with Glycos...
Jorge, Helena; Duarte, Isabel C.; Paiva, Sandra; Relvas, Ana Paula; Castelo-Branco, MiguelPublished online: March 22, 2022; Background: Risky health decisions and impulse control profiles may impact on metabolic control in type 1 diabetes mellitus (T1DM). We hypothesize that the neural correlates of cognitive impulsivity and decision-making in T1DM relate to metabolic control trajectories. Methods: We combined functional magnetic resonance imaging (fMRI), measures of metabolic trajectories (glycosyl...
Interactions of cytosolic tails in the Jen1 carboxylate transporter are critica...
Barata-Antunes, Cláudia; Talaia, Gabriel; Broutzakis, George; Ribas, David; De Beule, Pieter; Casal, Margarida; Stefan, Christopher J.Plasma membrane (PM) transporters of the major facilitator superfamily (MFS) are essential for cell metabolism, growth and response to stress or drugs. In Saccharomyces cerevisiae, Jen1 is a monocarboxylate/H+ symporter that provides a model to dissect the molecular details underlying cellular expression, transport mechanism and turnover of MFS transporters. Here, we present evidence revealing novel roles of th...
Yeast plasma membrane Fungal Oligopeptide Transporters display distinct substra...
Becerra-Rodríguez, Carmen; Taghouti, Géraldine; Portier, Perrine; Dequin, Sylvie; Casal, Margarida; Paiva, Sandra; Galeote, VirginieFungal Oligopeptide Transporters (Fot) Fot1, Fot2 and Fot3 have been found in <i>Saccharomyces cerevisiae</i> wine strains, but not in strains from other environments. In the <i>S. cerevisiae</i> wine strain EC1118, Fot1 and Fot2 are responsible for a broader range of oligopeptide utilization in comparison with strains not containing any Fot. This leads to better fermentation efficiency and an increased product...
Endocytosis of nutrient transporters in fungi: the ART of connecting signaling ...
Barata-Antunes, Cláudia; Alves, R.; Talaia, Gabriel; Casal, Margarida; Gerós, H.; Mans, Robert; Paiva, SandraPlasma membrane transporters play pivotal roles in the import of nutrients, including sugars, amino acids, nucleobases, carboxylic acids, and metal ions, that surround fungal cells. The selective removal of these transporters by endocytosis is one of the most important regulatory mechanisms that ensures a rapid adaptation of cells to the changing environment (e.g., nutrient fluctuations or different stresses). ...