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Internal Validation of the Investigator 26Plex QS Amplification Kit: a high-thr...
Neves Cardoso, Paula Liliana; Serra, Armando; Bogas, Vanessa; Balsa, Filipa; Lopes, Virginia; Dario, Rita; Porto, Maria João; Amorim, AntónioHuman singularity provides a foundation for forensic genetics, where the study of Short Tandem Repeats (STRs) is key. Furthermore, the development of polymerase chain reaction (PCR) has revolutionized the field by enabling the amplification of specific DNA markers. The Investigator® 26plex QS Amplification Kit is a highly robust multiplex PCR system, for human identification. In this study, we report the result...
Internal Validation of the Investigator 26Plex QS Amplification Kit: a high-thr...
Cardoso, Paula; Serra, Armando; Bogas, Vanessa; Balsa, Filipa; Lopes, Virginia; Dario, Rita; Porto, Maria João; Amorim, António; Corte Real, F.The Investigator® 26plex QS Amplification Kit, from QIAGEN, provides reliable and rapid DNA profiles while enabling the multiplex amplification of 24 STRs, 2 Quality Sensors and a gender-determining marker, Amelogenin. The Quality Sensors incorporated in this kit provide insight into the sample quality and the PCR's success while alerting to the presence of inhibitors. Through an extensive internal validation s...