6 documents found, page 1 of 1
Evaluation of bioenergetic and mitochondrial function in liver transplantation
Martins, Rui Miguel; Teodoro, João S.; Furtado, Emanuel; Rolo, Anabela Pinto; Palmeira, Carlos Marques; Tralhão, José GuilhermeBackground/Aims: We measured changes in mitochondrial function and bioenergetics that occur during ischemia/ reperfusion in fresh liver samples of patients undergoing liver transplantation. These variations correlated with markers of liver function and clinical outcome. Ischemia/reperfusion injury related to liver transplantation affects mitochondrial function and bioenergetics. Experimental studies were conduc...
Mild hypothermia during the reperfusion phase protects mitochondrial bioenerget...
Martins, Rui Miguel; Teodoro, João S.; Furtado, Emanuel; Oliveira, Rui Caetano; Tralhão, José Guilherme; Rolo, Anabela Pinto; Palmeira, Carlos MarquesThe organ preservation paradigm has changed following the development of new ways to preserve organs. The use of machine perfusion to preserve organs appears to have several advantages compared with conventional static cold storage. For liver transplants, the temperature control provided by machine perfusion improves organ preservation. In this experimental study, we measured the effects of different temperatur...
Recent insights into mitochondrial targeting strategies in liver transplantation
Martins, Rui Miguel; Teodoro, João Soeiro; Furtado, Emanuel; Rolo, Anabela Pinto; Palmeira, Carlos Marques; Tralhão, José GuilhermeIschemia/reperfusion (I/R) injury in liver transplantation can disrupt the normal activity of mitochondria in the hepatic parenchyma. This potential dysfunction of mitochondria after I/R injury could be responsible for the initial poor graft function or primary nonfunction observed after liver transplantation. Thus, determining the mechanisms that lead to human hepatic mitochondrial dysfunction might contribute...
Addition of Berberine to Preservation Solution in an Animal Model of Ex Vivo Li...
Martins, Rui Miguel; Rolo, Anabela Pinto; Teodoro, João Soeiro; Furtado, Emanuel; Oliveira, Rui Caetano; Tralhão, José GuilhermeLiver transplantation is a therapeutic regimen to treat patients with non-malignant end-stage liver diseases and malignant tumors of hepatic origin. The ischemia/reperfusion (I/R) injury in liver transplantation is associated with disruption of mitochondrial function in the hepatic parenchyma. Several studies have been conducted in animal models to identify pharmacological therapeutic strategies to minimize the...
Estimating gluconeogenesis by NMR isotopomer distribution analysis of [13 C]bic...
Alves, Tiago Cardoso; Nunes, Patrícia Maria; Palmeira, Carlos Marques; Jones, John Griffith; Carvalho, Rui AlbuquerqueThe gluconeogenic contribution to glucose production in livers isolated from rats fasted for 24 h was determined by 13C-NMR isotopomer distribution analysis of secreted glucose enriched from 99% [13C]bicarbonate (n = 4) and 99% [1-13C]lactate (n = 4). Experiments with 3% 2H2O were also performed, allowing the gluconeogenic contribution to be measured by the relative 2H enrichments at positions 5 and 2 of glucos...
Diabetes and mitochondrial oxidative stress: A study using heart mitochondria f...
Santos, Dario Loureiro; Palmeira, Carlos Marques; Seiça, Raquel; Dias, José; Mesquita, José; Moreno, Antonio Joaquim; Santos, Maria SanchaIncreasing evidence shows that the overproduction of reactive oxygen species, induced by diabetic hyperglycemia, contributes to the development of several cardiopathologies. The susceptibility of diabetic hearts to oxidative stress, induced in vitro by ADP-Fe2+ in mitochondria, was studied in 12-month-old Goto-Kakizaki rats, a model of non-insulin dependent diabetes mellitus, and normal (non-diabetic) Wistar ra...