27 documents found, page 1 of 3
Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly...
Esteves, Filipa; Vilhena Catarino Brito, David; Rajado, Ana Teresa; Silva, Nádia; Apolónio, Joana; Roberto, Vânia; Andrade, Raquel; Calado, SofiaHuman induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (h...
Investigator-Led Clinical Research in Portugal: Problem Identification and Prop...
Ferreira, João Pedro; Leite-Moreira, Adelino; da Costa-Pereira, Altamiro; Soares, António José; Robalo-Cordeiro, Carlos; Jerónimo, CármenN/a.; N/a.
Investigação clínica da iniciativa do investigador em Portugal: identificação d...
Ferreira, João Pedro; Leite-Moreira, Adelino; Da Costa-Pereira, Altamiro; Soares, António José; Robalo-Cordeiro, Carlos; Jerónimo, CármenPortugal tem um problema crónico de subfinanciamento da investigação científica. Apesar do investimento nacional ter vindo a aumentar nos últimos anos, este ainda é manifestamente insuficiente. A Fundação para a Ciência e Tecnologia (FCT) financia anualmente projetos de investigação em todas as áreas científicas, limitando o valor máximo a €250 000 por projeto. Todavia, a área científica na qual a investigação ...
Investigação clínica da iniciativa do investigador em Portugal: identificação d...
Ferreira, João Pedro; Leite-Moreira, Adelino; Da Costa-Pereira, Altamiro; Soares, António José; Robalo-Cordeiro, Carlos; Jerónimo, CármenPortugal tem um problema cronico de subfinanciamento da investigacao cientifica. Apesar do investimento nacional ter vindo a aumentar nos ultimos anos, este ainda e manifestamente insuficiente.1 A Fundacao para a Ciencia e Tecnologia (FCT) financia anualmente projetos de investigacao em todas as areas cientificas, limitando o valor maximo a euro250 000 por projeto. Todavia, a area cientifica na qual a investiga...
Reprogramming iPSCs to study age-related diseases: models, therapeutics, and cl...
Esteves, Filipa; Brito, David; Rajado, Ana Teresa; Silva, Nádia; Apolónio, Joana; Roberto, Vania Palma; Araújo, Inês Maria; Nóbrega, ClévioThe unprecedented rise in life expectancy observed in the last decades is leading to a global increase in the ageing population, and age-associated diseases became an increasing societal, economic, and medical burden. This has boosted major efforts in the scientific and medical research communities to develop and improve therapies to delay ageing and age-associated functional decline and diseases, and to expand...
rdml: A Mathematica package for parsing and importing Real-Time qPCR data
Magno, Ramiro; Duarte, Isabel; Andrade, Raquel P.; Palmeirim, IsabelObjective The purpose and objective of the research presented is to provide a package for easy importing of Real-Time PCR data markup language (RDML) data to Mathematica. Results Real-Time qPCR is the most widely used experimental method for the accurate quantification of gene expression. To enable the straightforward archiving and sharing of qPCR data and its associated experimental information, an XML-based d...
Mechanisms of vertebrate embryo segmentation: Common themes in trunk and limb d...
Sheeba, Caroline J.; Andrade, Raquel P.; Palmeirim, IsabelVarious ultradian rhythms ensure proper temporal regulations during embryo development. The embryo molecular clock, which was first identified in the presomitic mesoderm (PSM) underlying periodic somite formation, is one among them. Somites are the earliest manifestation of the segmented vertebrate body and they are formed with strict temporal precision. The tetrapod limb is also a segmented structure and the f...
Mechanisms of vertebrate embryo segmentation: Common themes in trunk and limb d...
Sheeba, C.J.; Andrade, Raquel P.; Palmeirim, IsabelVarious ultradian rhythms ensure proper temporal regulations during embryo development. The embryo molecular clock, which was first identified in the presomitic mesoderm (PSM) underlying periodic somite formation, is one among them. Somites are the earliest manifestation of the segmented vertebrate body and they are formed with strict temporal precision. The tetrapod limb is also a segmented structure and the f...
Getting a handle on embryo limb development: Molecular interactions driving lim...
Sheeba, C.J.; Andrade, Raquel P.; Palmeirim, IsabelDevelopment of the vertebrate embryo involves multiple segmentation processes to generate a functional, articulated organism. Cell proliferation, differentiation and patterning involve spatially and temporally regulated gene expression and signal transduction mechanisms. The developing vertebrate limb is an excellent model to study such fine-tuned regulations, whereby cells proliferate and are differentially sc...
Patterning in time and space: HoxB cluster gene expression in the developing ch...
Gouveia, Analuce Canha; Marcelino, Hugo Miguel Rocha; Gonçalves, Lisa; Palmeirim, Isabel; Andrade, Raquel P.The developing embryo is a paradigmatic model to study molecular mechanisms of time control in Biology. Hox genes are key players in the specification of tissue identity during embryo development and their expression is under strict temporal regulation. However, the molecular mechanisms underlying timely Hox activation in the early embryo remain unknown. This is hindered by the lack of a rigorous temporal frame...