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High prevalence of the simultaneous excretion of polyomaviruses JC and BK in th...
Nali, Luiz Henrique da Silva; Centrone, Cristiane de Campos; Urbano, Paulo Roberto Palma; Penalva-de-Oliveira, Augusto César; Vidal, Jose ErnestoOBJECTIVE: To evaluate the prevalence of the urinary excretion of BKV and JCV in HIV-infected patients without neurological symptoms. METHODS: Urine samples from HIV-infected patients without neurological symptoms were tested for JC virus and BK virus by PCR. Samples were screened for the presence of polyomavirus with sets of primers complementary to the early region of JCV and BKV genome (AgT). The presence of...
Comparison of direct immunofluorescence, conventional cell culture and polymera...
Pannuti, Claudio Sérgio
Comparison of direct immunofluorescence, conventional cell culture and polymera...
Reis, Alexanda Dias; Fink, Maria Cristina Domingues; Machado, Clarisse Martins; Paz Jr., José de Paula; Oliveira, Renato Reis; Tateno, Adriana FumieUm total de 316 amostras de lavado de nasofaringe obtidas de crianças em acompanhamento ambulatorial com até dois anos de idade durante episódio de doença aguda do trato respiratório foram processadas para detecção do vírus sincicial respiratório (VSR) utilizando três diferentes técnicas: isolamento viral, imunofluorescência direta e reação em cadeia por polimerase (RT-PCR). Destas amostras, 36 (11,4%) foram po...
Infecção pelo citomegalovírus em pacientes com síndrome da imunodeficiência adq...
Turchi, Marília Dalva; Pannuti, Claudio Sérgio; Sumita, Laura Masami; Vilas Boas, Lucy Santos; Weinberg, Adriana; Stavale, João NorbertoBetween April 1986 and June 1987, 50 patients meeting the CDC criteria for AIDS were studied for serological and virological evidence of CMV infection. Attempts for virus isolation from peripheral blood, urine and saliva were performed in cell culture lines of human foreskin fibroblasts and CMV specific IgG and IgM were assayed by IFI and IgG by ELISA. A total of 121 blood, 119 urine and 96 saliva samples were ...