4 documents found, page 1 of 1
Diarréia em bezerros da raça Nelore criados extensivamente: estudo clínico e et...
Oliveira Filho, José Paes de [UNESP]; Silva, Daniel P.G.; Pacheco, Marcelo D.; Mascarini, Luciene M. [UNESP]; Ribeiro, Márcio Garcia [UNESP]Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:50:40Z No. of bitstreams: 1 S0100-736X2007001000006.pdf: 102168 bytes, checksum: 3f9922581a04315b255b6bd7171fade0 (MD5); Made available in DSpace on 2013-08-22T18:50:40Z (GMT). No. of bitstreams: 1 S0100-736X2007001000006.pdf: 102168 bytes, checksum: 3f9922581a04315b255b6bd7171fade0 (MD5) Previous issue date: 2007-10-01; Made availabl...
Reproductive performance evaluation of a beef cattle herd naturally infected wi...
Junqueira, José Ricardo C.; Freitas, Júlio C. de; Alfieri, Alice F.; Alfieri, Amauri A.The aim of this study was the evaluation of some reproductive rates of a beef cattle herd that were naturally infected by the bovine herpesvirus 1 (BoHV-1), bovine viral diarrhea virus (BVDV) and Leptospira hardjo. In a herd with 1331 females, we randomly selected 208 animals that were distributed in three groups: i) 60 virgin heifers; ii) 60 first suckling cows; iii) 88 cows with two or more sucklings. All gro...
Comparison of different protocols for the bovine viral diarrhea virus detection...
Pilz, Daniela; Alfieri, Alice F.; Alfieri, Amauri A.The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV) from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV. To optimization of technique, the following conditions were evaluated: i) two pairs of primers, 103 / 372 (WEINSTOCK et al., 2001) and 324 / 326 (VILCEK et al., 1994)...
Development and evaluation of an enzyme-linked immunosorbent assay for the sero...
Ferreira, Melissa C.; Médici, Kerlei C.; Alfieri, Alice F.; Alfieri, Amauri A.An indirect enzyme-linked immunosorbent assay (ELISA) using non-purified antigen was developed for serological diagnosis of the bovine herpesvirus 1 (BoHV-1) infection. Some blocking substances to prevent nonspecific reactions were evaluated in different concentrations and combinations. The best results were obtained using 5% skim milk powder as blocking solution and the serum/conjugated buffer added with 10% M...