18 documents found, page 1 of 2
Guiding stem cell tenogenesis by modulation of growth factor signaling and cell...
Teixeira, Simão P. B.; Pardo, Alberto; Bakht, Syeda M.; Gómez-Florit, Manuel; Reis, R. L.; Gomes, Manuela E.; Domingues, Rui Miguel AndradeTendon injuries and tendinopathies are increasingly prevalent health problems currently lacking effective treatments. Tissue engineering offers promising strategies to boost the low innate regenerative ability of tendons. Within this context, the simultaneous leveraging of both physical and biochemical cues by engineered scaffolding systems can be explored to promote a stronger tenogenic response from stem cell...
Combining magnetically-assisted and matrix-assisted 3D bioprinting for anisotro...
Bakht, Syeda M.; Pardo, Alberto; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.A major obstacle in biofabrication is replicating the organization of the extracellular matrix and cellular patterns found in anisotropic tissues within bioengineered constructs. While magnetically-assisted 3D bioprinting techniques have the potential to create scaffolds that mimic natural biological structures, they currently lack the ability to accurately control the dispersion of magnetic substances within t...
3D biomimetic constructs steer stem cell commitment by synergistic modulation o...
Teixeira, Simão P. B.; Pardo, Alberto; Bakht, Syeda M.; Gómez-Florit, Manuel; Reis, R. L.; Gomes, Manuela E.; Domingues, Rui Miguel AndradeTendon diseases are prevalent health concerns for which current therapies present limited success, in part due to the intrinsically low regenerative ability of tendons. Therefore, tissue engineering presents a potential to improve this outcome. Here, we hypothesize that a concurrent control over both biophysical and biochemical stimuli will boost the tenogenic commitment of stem cells, thus promoting regenerati...
Hierarchical design of fibrous tissue-mimetic scaffolds
Pardo, Alberto; Gómez-Florit, Manuel; Davidson, Matthew D; Öztürk-Öncel, M. Özgen; Domingues, Rui Miguel Andrade; Burdick, Jason A..; Gomes, Manuela E.Most tissues of the human body present hierarchical fibrillar extracellular matrices that have a strong influence over their physicochemical properties and biological behavior. Of great interest is the introduction of this fibrillar structure to hydrogels, particularly due to the water-rich composition, cytocompatibility and tunable properties of this class of biomaterials. Here, the main bottom-up fabrication ...
Human tendon-on-chip: unveiling the effect of core compartment-t cell spatiotem...
Bakht, Syeda M.; Pardo, Alberto; Gómez-Florit, Manuel; Caballero, David; Kundu, Subhas C; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.The lack of representative in vitro models recapitulating human tendon (patho)physiology is among the major factors hindering consistent progress in the knowledge-based development of adequate therapies for tendinopathy.Here, an organotypic 3D tendon-on-chip model is designed that allows studying the spatiotemporal dynamics of its cellular and molecular mechanisms.Combining the synergistic effects of a bioactiv...
Tabulation with Zippers
Viera, Marcos; Pardo, Alberto; Saraiva, JoãoTabulation is a well-known technique for improving the efficiency of recursive functions with redundant function calls. A key point in the application of this technique is to identify a suitable representation for the table. In this paper, we propose the use of zippers as tables in the tabulation process. Our approach relies on a generic function zipWithZipper, that makes strong use of lazy evaluation to traver...
Magnetically-assisted 3D bioprinting of anisotropic tissue-mimetic constructs
Pardo, Alberto; Bakht, Syeda Mahwish; Gomez-Florit, Manuel; Rial, Ramón; Monteiro, Rosa Conceiçao Freitas; Teixeira, Simão P. B.; Taboada, PabloRecreating the extracellular matrix organization and cellular patterns of aniso-tropic tissues in bioengineered constructs remains a significant biofabrication challenge. Magnetically-assisted 3D bioprinting strategies can be exploited to fabricate biomimetic scaffolding systems, but they fail to provide control over the distribution of magnetic materials incorporated in the bioinks while pre-serving the fideli...
Magnetic nanocomposite hydrogels for tissue engineering: design concepts and re...
Pardo, Alberto; Gomez-Florit, Manuel; Barbosa, Silvia; Taboada, Pablo; Domingues, Rui Miguel Andrade; Gomes, Manuela E.Most tissues of the human body are characterized by highly anisotropic physical properties and biological organization. Hydrogels have been proposed as scaffolding materials to construct artificial tissues due to their water-rich composition, biocompatibility and tunable properties. However, unmodified hydrogels are typically composed of randomly oriented polymer networks, resulting in homogeneous structures wi...
Natural-based hydrogels for tissue engineering applications
Gomez-Florit, Manuel; Pardo, Alberto; Domingues, Rui Miguel Andrade; Graça, Ana L.; Babo, Pedro Miguel Sousa; Reis, R. L.; Gomes, Manuela E.In the field of tissue engineering and regenerative medicine, hydrogels are used as biomaterials to support cell attachment and promote tissue regeneration due to their unique biomimetic characteristics. The use of natural-origin materials significantly influenced the origin and progress of the field due to their ability to mimic the native tissuesâ extracellular matrix and biocompatibility. However, the majori...
Memoized zipper-based attribute grammars and their higher order extension
Fernandes, João Paulo; Martins, Pedro; Pardo, Alberto; Saraiva, João; Viera, MarcosAttribute grammars are a powerfull, well-known formalism to implement and reason about programs which, by design, are conveniently modular. In this work we focus on a state of the art zipper-based embedding of classic attribute grammars and higher-order attribute grammars. We improve their execution performance through controlling attribute (re)evaluation by means of memoization techniques. We present the resul...