33 documents found, page 1 of 4
Epidemiological, behavioural and molecular profile of individuals newly diagnos...
da Veiga Leal, Silvânia; de Pina Araújo, Isabel Inês; Pimentel, Victor; Parreira, Ricardo; Gonçalves, Paloma; Pingarilho, Marta; Taveira, NunoIntroduction: The diagnosis of HIV with Transmitted Drug Resistance Mutations (TDR) limits therapeutic options and consequently complicates the management of HIV-1 cases. Objective: The aim of this study was to describe the molecular epidemiological profile and TDR in individuals newly diagnosed with HIV-1 from 2018 to 2020. Materials and methods: The HIV-1 pol gene was amplified and sequenced using the Sanger ...
Ticks and Tick-Borne Pathogens Circulating in Peri-Domestic Areas in Mainland P...
Moerbeck, Leonardo; Parreira, Ricardo; Szczotko, Magdalena; Seixas, Gonçalo; Velez, Rita; Dmitryjuk, Małgorzata; Santos, Ana Sofia; Domingos, AnaOver the years, tick-borne pathogens (TBPs) have garnered significant interest due to their medical, veterinary and economic importance. Additionally, TBPs have drawn attention to how these microorganisms interact with their own vectors, increasing the risk to human and animal infection of emerging and reemerging zoonoses. In this sense, ticks, which are obligate hematophagous ectoparasites, have a key role in ...
High-Sensitivity RT-LAMP for Molecular Detection of O’nyong-nyong (Alphavirus o...
Faísca-Silva, David; Seixas, Gonçalo; M., Nunes; Parreira, RicardoMosquitoes serve as vectors for many arthropod-borne viruses (arboviruses) that are responsible for millions of human infections and thousands of deaths each year. Among these arboviruses, O’nyong-nyong virus (ONNV) is an African alphavirus mainly transmitted by Anopheles mosquitoes. ONNV can be detected through serological or molecular tests, the first showing cross-reactivity to co-circulating alphaviruses an...
Perfil epidemiológico, comportamental e molecular de indivíduos recém-diagnosti...
Leal, Silvânia da Veiga; Araújo, Isabel Inês de Pina; Pimentel, Victor; Parreira, Ricardo; Gonçalves, Paloma; Pingarilho, Marta; Taveira, NunoIntrodução: O diagnóstico de VIH com Mutações de Resistência Transmitida (TDR) limita a opção terapêuticas e consequentemente dificulta a gestão de casos VIH-1. Objetivo: Este artigo tem como objetivo descrever o perfil epidemiológico molecular e de TDR em indivíduos com diagnóstico recente de VIH-1, no período de 2018 a 2020. Materiais e métodos: O gene pol VIH-1 foi amplificado e sequenciado pelo método Sange...
Complete blood count parameters as biomarkers of retinopathy of prematurity: a ...
Fevereiro-Martins, Mariza; Santos, Ana Carolina; Marques-Neves, Carlos; Guimarães, Hercília; Bicho, Manuel; Afonso, Conceição; Ferreira, JoanaPurpose: To evaluate complete blood count (CBC) parameters in the first week of life as predictive biomarkers for the development of retinopathy of prematurity (ROP). Methods: Multicenter, prospective, observational study of a cohort of preterm infants born with gestational age (GA) < 32 weeks or birth weight < 1500 g in eight Portuguese neonatal intensive care units. All demographic, clinical, and laboratory d...
Práticas de Rastreio e Tratamento da Retinopatia da Prematuridade em Portugal
Vieira, Maria João; Miranda, Vasco; Parreira, Ricardo; Maia, Sofia; Caiado, Filipa; Menéres, Pedro; Sousa, João PauloOBJETIVOS: O rastreio e tratamento da retinopatia da prematuridade (ROP) varia significa-tivamente entre unidades hospitalares. O objetivo deste estudo é sintetizar as práticas atuais no rastreio, diagnóstico e tratamento da ROP em Portugal, MATERIAL E MÉTODOS: Os oftalmologistas portugueses com prática no rastreio de ROP foram con-vidados a preencher um questionário online anónimo relacionado com o rastreio, t...
Paralisia Traumática Bilateral do Nervo Abducente
Marta, Ana; Maia, Sofia; Miranda, Vasco; Parreira, Ricardo; Menéres, PedroIntrodução: Nosso objetivo é relatar um caso de paralisia traumática grave do VI par bilateral. Descrevemos um caso clínico documentado com fotografias em diferentes posições do olhar; gráficos do ecrã de Hess e testes de campo visual de Goldmann durente o período de seguimento. Relato de caso: Um homem de 42 anos envolveu-se num acidente de carro de alta energia (240 km/h). Nove dias depois, quando foi transfe...
Giardia duodenalis infection in dogs from the metropolitan area of Lisbon, Port...
Pereira, André; Teixeira, Joana; Sousa, Sofia; Parreira, Ricardo; Campino, Lenea; Meireles, José; Maia, CarlaGiardia duodenalis is a cosmopolitan enteric protozoan that affects a wide range of vertebrates, including humans and dogs. Genetic characterisation reveals eight different assemblages, with A and B having been found mainly in humans and several other animals, and thus considered potentially zoonotic, while C and D are adapted to infect dogs. This study aimed to determine the prevalence of G. duodenalis, their ...
Genetic Diversity of norA, Coding for a Main Efflux Pump of Staphylococcus aureus
Santos Costa, Sofia; Sobkowiak, Benjamin; Parreira, Ricardo; Edgeworth, Jonathan D.; Viveiros, Miguel; Clark, Taane G.; Couto, IsabelFunding Information: This work was partially supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal), through funds to GHTM – UID/Multi/04413/2013. SC was supported by grant SFRH/BPD/97508/2013 from FCT, Portugal. TC was funded by the Medical Research Council United Kingdom (Grant Nos. MR/K000551/1, MR/M01360X/1, MR/N010469/1, and MR/R020973/1) and BBSRC United Kingdom (BB/R013063/1). BS was funded ...
A diverse assemblage of RNA and DNA viruses found in mosquitoes collected in so...
Silva, Manuel; Morais, Paulo; Maia, Carla; Sousa, Carolina Bruno de; Gouveia de Almeida, Antonio Paulo; Parreira, RicardoThis work describes the detection and partial characterization of mosquito-borne virus genomic sequences, based on the analysis of mosquitoes collected from the Spring to Fall of 2018 in the Algarve (southern Portugal). The viral survey that was carried out using multiple primer sets disclosed the presence of both RNA and DNA viral sequences in these mosquitoes, which were subsequently analysed using maximum li...