7 documents found, page 1 of 1
Hybrid next-generation sequencing protocol for testing HIV-2 drug resistance
Gonçalves, Fátima; Cabanas, Joaquim; Costa, Inês; Veloso, Margarida; Ribeiro, Marta; Fernandes, Sandra; Diogo, Isabel; Sebastião, Cruz S.Funding Information: Researchers from GHTM|IHMT, CNIC, CISA, or INIS received financial support from FCT-Foundation for Science and Technology (MARVEL project - PTDC/SAU-PUB/4018/2021), FCG-Calouste Gulbenkian Foundation (HITOLA project - N\u00BA.250466), PDCT-Science and Technology Development Project (N\u00BA.36 MESCTI/PDCT/2022), GHTM-UID/04413/2020 and LA-REAL-LA/P/0117/2020. Egas Moniz Center for Interdisc...
Applying Next-Generation Sequencing to Track HIV-1 Drug Resistance Mutations Ci...
Pimentel, Victor; Pingarilho, Marta; Sebastião, Cruz S.; Miranda, Mafalda; Gonçalves, Fátima; Cabanas, Joaquim; Costa, Inês; Diogo, IsabelFunding Information: This research was funded by FCT in the Scopus of INTEGRIV project (PTDC/SAU-INF/31990/2017), FCT MARVEL (PTDC/SAU-PUB/4018/2021), Calouste Gulbenkian Foundation (FCG), under the ENVOLVE Ci\u00EAncia PALOP program that funded the HITOLA project (N\u00BA.250466), Africa Research Excellence Fund (AREF) (AREF-312-CRUZ-F-C0931), Science and Technology Development Project (PDCT) within the scope ...
Differential patterns of post-migration HIV-1 infection acquisition among Portu...
Pimentel, Victor Figueiredo; Pingarilho, Marta; Sole, Giordano; Alves, Daniela; Miranda, Mafalda; Diogo, Isabel; Fernandes, Sandra; Pineda-pena, AndreaOBJECTIVE: To investigate the dynamics of phylogenetic transmission clusters involving immigrants of Portuguese Speaking Countries living in Portugal. DESIGN/METHODS: We included genomic sequences, sociodemographic and clinical data from 772 HIV migrants followed in Portugal between 2001 and 2017. To reconstruct HIV-1 transmission clusters, we applied phylogenetic inference from 16 454 patients: 772 migrants, 2...
Increasing prevalence of HIV-1 transmitted drug resistance in Portugal
Pingarilho, Marta; Pimentel, Victor; Diogo, Isabel; Fernandes, Sandra; Miranda, Mafalda; Pineda-Peña, AC; Libin, Pieter; Theys, Kristof; Martins, MRIntroduction: Treatment for All recommendations have allowed access to antiretroviral (ARV) treatment for an increasing number of patients. This minimizes the transmission of infection but can potentiate the risk of transmitted (TDR) and acquired drug resistance (ADR). Objective: To study the trends of TDR and ADR in patients followed up in Portuguese hospitals between 2001 and 2017. Methods: In total, 11,911 p...
Molecular epidemiology of HIV-1 infected migrants followed up in Portugal
Pimentel, Victor; Pingarilho, Marta; Alves, Daniela; Diogo, Isabel; Fernandes, Sandra; Miranda, Mafalda; Pineda-Peña, Andrea Clemencia; Libin, PieterMigration is associated with HIV-1 vulnerability. Objectives: To identify long-term trends in HIV-1 molecular epidemiology and antiretroviral drug resistance (ARV) among migrants followed up in Portugal Methods: 5177 patients were included between 2001 and 2017. Rega, Scuel, Comet, and jPHMM algorithms were used for subtyping. Transmitted drug resistance (TDR) and Acquired drug resistance (ADR) were defined as ...
Characterization of a large cluster of HIV-1 A1 infections detected in Portugal...
Araújo, Pedro M.M.; Carvalho, Alexandre; Pingarilho, Marta; Faria, Domitília; Pinho, Raquel; Ferreira, José; Proença, Paula; Nunes, SofiaHIV-1 subtypes associate with differences in transmission and disease progression. Thus, the existence of geographic hotspots of subtype diversity deepens the complexity of HIV-1/AIDS control. The already high subtype diversity in Portugal seems to be increasing due to infections with sub-subtype A1 virus. We performed phylogenetic analysis of 65 A1 sequences newly obtained from 14 Portuguese hospitals and 425 ...
Characterization of NS5A and NS5B Resistance-Associated Substitutions from Geno...
Ruben, Brandão,; Rute, Marcelino,; Goncalves, Maria de Fátima; Diogo, Isabel; Carvalho, A P; Cabanas, Joaquim; Costa, Inês; Brogueira, Pedro; Ventura, FThis study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all G...